ArticleGenetics2010
Discovery of mutations in Saccharomyces cerevisiae by pooled linkage analysis and whole-genome sequencing.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed.
- A lethal ORC ATPase mutation is suppressed by alterations in ORC and RNA Pol II transcription components.PloS one · 2026Article
- Thiolutin has complex effects in vivo but is a direct inhibitor of RNA polymerase II in vitro.Nucleic acids research · 2024Article
- Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere.Communications biology · 2023Article
- Contributions of Adaptive Laboratory Evolution towards the Enhancement of the Biotechnological Potential of Non-Conventional Yeast Species.Journal of fungi (Basel, Switzerland) · 2023Review
- A QTL on chromosome IV explains a natural variation of QR.pap final position inmicroPublication biology · 2023Article
- Anterior-posterior pattern formation in ciliates.The Journal of eukaryotic microbiology · 2022Review
- Bur1 functions with TORC1 for vacuole-mediated cell cycle progression.EMBO reports · 2022Article
- Essentiality of Sis1, a J-domain protein Hsp70 cochaperone, can be overcome by Tti1, a specialized PIKK chaperone.Molecular biology of the cell · 2022Article
- Essential histone chaperones collaborate to regulate transcription and chromatin integrity.Genes & development · 2021Article
- A Major Effect Gene Controlling Development and Pathogenicity inFrontiers in plant science · 2021Article
- Metataxonomic Analysis of Grape Microbiota During Wine Fermentation Reveals the Distinction of Cyprus RegionalFrontiers in microbiology · 2021Article
- The role of gene dosage in budding yeast centrosome scaling and spontaneous diploidization.PLoS genetics · 2020Article
- MutantHuntWGS: A Pipeline for IdentifyingG3 (Bethesda, Md.) · 2020Article
- Spontaneous mutations that confer resistance to 2-deoxyglucose act through Hxk2 and Snf1 pathways to regulate gene expression and HXT endocytosis.PLoS genetics · 2020Article
- A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin.eLife · 2020Article
- Factors promoting nuclear envelope assembly independent of the canonical ESCRT pathway.The Journal of cell biology · 2020Article
- Loss of vacuolar acidity results in iron-sulfur cluster defects and divergent homeostatic responses during aging in Saccharomyces cerevisiae.GeroScience · 2020Article
- Fork pausing complex engages topoisomerases at the replisome.Genes & development · 2020Article
- A Novel Factor Essential for Unconventional Secretion of Chitinase Cts1.Frontiers in microbiology · 2020Article
- Whole-Genome Sequencing of Yeast Cells.Current protocols in molecular biology · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Many novel and important mutations arise in model organisms and human patients that can be difficult or impossible to identify using standard genetic approaches, especially for complex traits. Working with a previously uncharacterized dominant Saccharomyces cerevisiae mutant with impaired vacuole inheritance, we developed a pooled linkage strategy based on next-generation DNA sequencing to specifically identify functional mutations from among a large excess of polymorphisms, incidental mutations, and sequencing errors. The VAC6-1 mutation was verified to correspond to PHO81-R701S, the highest priority candidate reported by VAMP, the new software platform developed for these studies. Sequence data further revealed the large extent of strain background polymorphisms and structural alterations present in the host strain, which occurred by several mechanisms including a novel Ty insertion. The results provide a snapshot of the ongoing genomic changes that ultimately result in strain divergence and evolution, as well as a general model for the discovery of functional mutations in many organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.