ArticleBiophysical journal2010
Improved probes for hybrid voltage sensor imaging.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 67 citations in OpenAlex.
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Distinct Patterns of Evoked Firing in Engram and Non-Engram Neurons in the Dentate Gyrus of Hippocampal Slices.Hippocampus · 2025Article
- Plasma membrane-endoplasmic reticulum coupling probed with genetically-encoded voltage sensors.bioRxiv : the preprint server for biology · 2025Article
- Fxr1 Deletion from Cortical Parvalbumin Interneurons Modifies Their Excitatory Synaptic Responses.eNeuro · 2025Article
- Intrinsic and Synaptic Contributions to Repetitive Spiking in Dentate Granule Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Inter and intralaminar excitation of parvalbumin interneurons in mouse barrel cortex.PloS one · 2024Article
- Velocity of conduction between columns and layers in barrel cortex reported by parvalbumin interneurons.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- Inter and Intralaminar Excitation of Parvalbumin Interneurons in Mouse Barrel Cortex.bioRxiv : the preprint server for biology · 2023Article
- Unitary synaptic responses of parvalbumin interneurons evoked by excitatory neurons in the mouse barrel cortex.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus.eNeuro · 2023Article
- Advancements in the Quest to Map, Monitor, and Manipulate Neural Circuitry.Frontiers in neural circuits · 2022Review
- Direct synaptic excitation between hilar mossy cells revealed with a targeted voltage sensor.Hippocampus · 2021Article
- Article
- Small Molecule-Protein Hybrid for Voltage Imaging via Quenching of Bioluminescence.ACS sensors · 2021Article
- Imaging the electrical activity of organelles in living cells.Communications biology · 2021Article
- A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.Micromachines · 2021Review
- Genetically Encoded Voltage Indicators.Advances in experimental medicine and biology · 2021Review
- Enhanced genetically encoded voltage indicators advance their applications in neuroscience.Current opinion in biomedical engineering · 2019Article
- Optical Studies of Action Potential Dynamics with hVOS probes.Current opinion in biomedical engineering · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Hybrid voltage sensors (hVoS) probe membrane potential by coupling the fluorescence of membrane-anchored proteins to the movement of a membrane-embedded hydrophobic anion dipicrylamine. Fluorescence resonance energy transfer between these two components transduces voltage changes into fluorescence changes, providing a signal for imaging electrical activity in genetically targeted cells. To improve hVoS signals, we systematically varied the optical properties, membrane targeting motifs, and linkages of fluorescent proteins to optimize the normalized fluorescence change (ΔF/F) and signal/noise ratio. The best results were obtained with cerulean fluorescent protein tagged N-terminally with a GAP43 motif and C-terminally with a truncated h-ras motif. With 100 mV steps in PC12 cells, this probe produced ΔF/F = 26% (4 μM dipicrylamine), which was threefold greater than that obtained with the original farnesylated EGFP construct. We also obtained a fivefold greater signal/noise ratio, which was 70% of a theoretical optimum. We designate this GAP43-CerFP-t-h-ras construct as hVoS 2.0. With the aid of a theoretical analysis, we estimated that hVoS 2.0 places its fluorophore ∼40 Å from the bilayer midplane. hVoS 2.0 performed well in cultured hippocampal neurons, where single action potentials produced clear fluorescence changes in a single trial. This improved probe should help investigators image voltage in genetically targeted neurons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.