ArticleBiophysical journal2010
Quantitative analysis of actin turnover in Listeria comet tails: evidence for catastrophic filament turnover.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Length control emerges from cytoskeletal network geometry.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Length control emerges from cytoskeletal network geometry.bioRxiv : the preprint server for biology · 2023Article
- Catastrophic actin filament bursting by cofilin, Aip1, and coronin.The Journal of biological chemistry · 2020Article
- Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation.Developmental cell · 2020Article
- CD2AP links actin to PI3 kinase activity to extend epithelial cell height and constrain cell area.The Journal of cell biology · 2020Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Arabidopsis CDPK6 phosphorylates ADF1 at N-terminal serine 6 predominantly.Plant cell reports · 2013Article
- Arabidopsis actin-depolymerizing factor7 severs actin filaments and regulates actin cable turnover to promote normal pollen tube growth.The Plant cell · 2013Article
- Mechanisms of actin disassembly.Molecular biology of the cell · 2013Article
- Cyclase-associated protein (CAP) acts directly on F-actin to accelerate cofilin-mediated actin severing across the range of physiological pH.The Journal of biological chemistry · 2012Article
- Analysis of tubulin alpha-1A/1B C-terminal tail post-translational poly-glutamylation reveals novel modification sites.Journal of proteome research · 2012Article
- Reconstitution of amoeboid motility in vitro identifies a motor-independent mechanism for cell body retraction.Current biology : CB · 2011Article
- Arabidopsis actin depolymerizing factor4 modulates the stochastic dynamic behavior of actin filaments in the cortical array of epidermal cells.The Plant cell · 2011Article
- Robust organizational principles of protrusive biopolymer networks in migrating living cells.PloS one · 2011Article
- Putative biomarkers and targets of estrogen receptor negative human breast cancer.International journal of molecular sciences · 2011Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Rapid assembly and disassembly (turnover) of actin filaments in cytoplasm drives cell motility and shape remodeling. While many biochemical processes that facilitate filament turnover are understood in isolation, it remains unclear how they work together to promote filament turnover in cells. Here, we studied cellular mechanisms of actin filament turnover by combining quantitative microscopy with mathematical modeling. Using live cell imaging, we found that actin polymer mass decay in Listeria comet tails is very well fit by a simple exponential. By analyzing candidate filament turnover pathways using stochastic modeling, we found that exponential polymer mass decay is consistent with either slow treadmilling, slow Arp2/3-dissociation, or catastrophic bursts of disassembly, but is inconsistent with acceleration of filament turnover by severing. Imaging of single filaments in Xenopus egg extract provided evidence that disassembly by bursting dominates isolated filament turnover in a cytoplasmic context. Taken together, our results point to a pathway where filaments grow transiently from barbed ends, rapidly terminate growth to enter a long-lived stable state, and then undergo a catastrophic burst of disassembly. By keeping filament lengths largely constant over time, such catastrophic filament turnover may enable cellular actin assemblies to maintain their mechanical integrity as they are turning over.
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