ArticleBiophysical journal2010
The role of amino terminus of mouse Cx50 in determining transjunctional voltage-dependent gating and unitary conductance.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Multi-ion permeation and dynamic conductance modulation in connexin gap junction channels.bioRxiv : the preprint server for biology · 2026Article
- Lipids intercalate and mediate multi-channel assemblies of connexin-46/50 gap junctions.bioRxiv : the preprint server for biology · 2026Article
- Reversible lipid-mediated pH-gating of connexin-46/50 by cryo-EM.Nature communications · 2026Article
- Genetically engineered human embryonic kidney cells as a novel vehicle for dual patch clamp study of human gap junction channels.The Biochemical journal · 2024Article
- Cx31.1 can selectively intermix with co-expressed connexins to facilitate its assembly into gap junctions.Journal of cell science · 2024Article
- Article
- The Hydrophobic Residues in Amino Terminal Domains of Cx46 and Cx50 Are Important for Their Gap Junction Channel Ion Permeation and Gating.International journal of molecular sciences · 2022Article
- Function and Plasticity of Electrical Synapses in the Mammalian Brain: Role of Non-Junctional Mechanisms.Biology · 2022Review
- Connexin 46 and connexin 50 gap junction channel properties are shaped by structural and dynamic features of their N-terminal domains.The Journal of physiology · 2021Article
- Structural determinants underlying permeant discrimination of the Cx43 hemichannel.The Journal of biological chemistry · 2019Article
- Gap junction structure: unraveled, but not fully revealed.F1000Research · 2017Review
- Exome sequencing identifies a novel mutation in GJA8 associated with inherited cataract in a Chinese family.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2017Article
- Exploring the Membrane Potential of Simple Dual-Membrane Systems as Models for Gap-Junction Channels.Biophysical journal · 2016Article
- Determinants of Cx43 Channel Gating and Permeation: The Amino Terminus.Biophysical journal · 2016Article
- A novel Cx50 (GJA8) p.H277Y mutation associated with autosomal dominant congenital cataract identified with targeted next-generation sequencing.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2015Article
- The First Extracellular Domain Plays an Important Role in Unitary Channel Conductance of Cx50 Gap Junction Channels.PloS one · 2015Article
- Charge at the 46th residue of connexin 50 is crucial for the gap-junctional unitary conductance and transjunctional voltage-dependent gating.The Journal of physiology · 2014Article
- An atrial-fibrillation-linked connexin40 mutant is retained in the endoplasmic reticulum and impairs the function of atrial gap-junction channels.Disease models & mechanisms · 2014Article
- Motifs in the permeation pathway of connexin channels mediate voltage and Ca (2+) sensing.Frontiers in physiology · 2014Review
- Functional roles of the amino terminal domain in determining biophysical properties of Cx50 gap junction channels.Frontiers in physiology · 2013Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Amino-terminus and carboxyl-terminus of connexins have been proposed to be responsible for the transjunctional voltage-dependent gating (V(j)-gating) and the unitary gap junction channel conductance (γ(j)). To better understand the molecular structure(s) determining the V(j)-gating properties and the γ(j) of Cx50, we have replaced part of the amino-terminus of mCx50 by the corresponding domain of mCx36 to engineer a chimera Cx50-Cx36N, and attached GFP at the carboxyl-terminus of mCx50 to construct Cx50-GFP. The dual whole-cell patch-clamp technique was used to test the resulting gap junction channel properties in N2A cells. The Cx50-Cx36N gap junction channel lowered the sensitivity of steady-state junctional conductance to V(j) (G(j)/V(j) relationship), slowed V(j)-gating kinetics, and reduced γ(j) as compared to Cx50 channel. Cx50-GFP gap junction channel showed similar V(j)-gating properties and γ(j) to Cx50 channel. We further characterized a mutation, Cx50N9R, where the Asn (N) at the ninth position of Cx50 was replaced by the corresponding Arg (R) at Cx36. The G(j)/V(j) relationship of Cx50N9R channel was significantly changed; most strikingly, the macroscopic residual conductance (G(min)) was near zero. Moreover, the single Cx50N9R channel only displayed one open state (γ(j) = 132 ± 4 pS), and no substate could be detected. Our data suggest that the NT of Cx50 is critical for both the V(j)-gating and the γ(j), and the introduction of a positively charged Arg at the ninth position reduced the G(min) with a correlated disappearance of the substate at the single channel level.
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