ArticleMolecular microbiology2010
Biochemical analysis of PIFTC3, the Trypanosoma brucei orthologue of nematode DYF-13, reveals interactions with established and putative intraflagellar transport components.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Identification of theMolecular syndromology · 2021Article
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- Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly.The EMBO journal · 2019Article
- Glucose Transporters and Virulence inmSphere · 2018Article
- IFT56 regulates vertebrate developmental patterning by maintaining IFTB complex integrity and ciliary microtubule architecture.Development (Cambridge, England) · 2017Article
- Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.PloS one · 2015Article
- The intraflagellar transport dynein complex of trypanosomes is made of a heterodimer of dynein heavy chains and of light and intermediate chains of distinct functions.Molecular biology of the cell · 2014Article
- The GTPase IFT27 is involved in both anterograde and retrograde intraflagellar transport.eLife · 2014Article
- Boarder control on the IFT train.eLife · 2014Article
- Article
- Article
- Trypanosoma brucei FKBP12 differentially controls motility and cytokinesis in procyclic and bloodstream forms.Eukaryotic cell · 2013Article
- Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain.PLoS genetics · 2013Article
- 'Transient' genetic suppression facilitates generation of hexose transporter null mutants in Leishmania mexicana.Molecular microbiology · 2013Article
- Independent analysis of the flagellum surface and matrix proteomes provides insight into flagellum signaling in mammalian-infectious Trypanosoma brucei.Molecular & cellular proteomics : MCP · 2011Article
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2 authors at 1 institution in 1 country.
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Abstract
DYF-13, originally identified in Caenorhabditis elegans within a collection of dye-filling chemosensory mutants, is one of several proteins that have been classified as putatively involved in intraflagellar transport (IFT), the bidirectional movement of protein complexes along cilia and flagella and specifically in anterograde IFT. Although genetic studies have highlighted a fundamental role of DYF-13 in nematode sensory cilium and trypanosome flagellum biogenesis, biochemical studies on DYF-13 have lagged behind. Here, we show that in Trypanosoma brucei the orthologue to DYF-13, PIFTC3, participates in a macromolecular complex of approximately 660 kDa. Mass spectroscopy of affinity-purified PIFTC3 revealed several components of IFT complex B as well as orthologues of putative IFT factors DYF-1, DYF-3, DYF-11/Elipsa and IFTA-2. DYF-11 was further analysed and shown to be concentrated near the basal bodies and in the flagellum, and to be required for flagellum elongation. In addition, by coimmunoprecipitation we detected an interaction between DYF-13 and IFT122, a component of IFT complex A, which is required for retrograde transport. Thus, our biochemical analysis supports the model, proposed by genetic analysis in C. elegans, that the trypanosome orthologue of DYF-13 plays a central role in the IFT mechanism.
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