ArticleACS chemical biology2010
A biosynthetic strategy for re-engineering the Staphylococcus aureus cell wall with non-native small molecules.
Article in ACS chemical biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Redirecting the host immune response to bacterial infection with antibody-recruiting molecules (ARMs).Current opinion in chemical biology · 2025Review
- Noninvasive Analysis of Peptidoglycan from Living Animals.Bioconjugate chemistry · 2024Article
- Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold.ACS chemical biology · 2022Article
- Genetic Determinants of Surface Accessibility inBioconjugate chemistry · 2022Article
- Natural and Synthetic Sortase A Substrates Are Processed byBioconjugate chemistry · 2022Article
- Chemical Reporters for Bacterial Glycans: Development and Applications.Chemical reviews · 2022Review
- SaccuFlow: A High-Throughput Analysis Platform to Investigate Bacterial Cell Wall Interactions.ACS infectious diseases · 2021Article
- β-Lactams against the Fortress of the Gram-PositiveChemical reviews · 2021Review
- From Differential Stains to Next Generation Physiology: Chemical Probes to Visualize Bacterial Cell Structure and Physiology.Molecules (Basel, Switzerland) · 2020Review
- A Cell-based Screen in Actinomyces oris to Identify Sortase Inhibitors.Scientific reports · 2020Article
- Decorating bacteria with self-assembled synthetic receptors.Nature communications · 2020Article
- A How-To Guide for Mode of Action Analysis of Antimicrobial Peptides.Frontiers in cellular and infection microbiology · 2020Review
- Sortase A Mediated Bioconjugation of Common Epitopes Decreases Biofilm Formation inFrontiers in microbiology · 2020Article
- Metabolic Incorporation of N-Acetyl Muramic Acid Probes into Bacterial Peptidoglycan.Current protocols in chemical biology · 2019Article
- Broadening the scope of sortagging.RSC advances · 2019Review
- Synthesis of Functionalized N-Acetyl Muramic Acids To Probe Bacterial Cell Wall Recycling and Biosynthesis.Journal of the American Chemical Society · 2018Article
- Synthetic Immunobiotics: A Future Success Story in Small Molecule-Based Immunotherapy?ACS infectious diseases · 2018Article
- Postsynthetic Modification of Bacterial Peptidoglycan Using Bioorthogonal N-Acetylcysteamine Analogs and Peptidoglycan O-Acetyltransferase B.Journal of the American Chemical Society · 2017Article
- Immuno-targeting ofChemical science · 2017Article
- Rapid and Direct VHH and Target Identification by Staphylococcal Surface Display Libraries.International journal of molecular sciences · 2017Article
Corrections and comments
- Erratum issuedKazmiercak, Barbara I [corrected to Kazmierczak, Barbara I]
Authors and funding
7 authors.
Funding
Abstract
Staphylococcus aureus (S. aureus) is a Gram-positive bacterial pathogen that has emerged as a major public health threat. Here we report that the cell wall of S. aureus can be covalently re-engineered to contain non-native small molecules. This process makes use of endogenous levels of the bacterial enzyme sortase A (SrtA), which ordinarily functions to incorporate proteins into the bacterial cell wall. Thus, incubation of wild-type bacteria with rationally designed SrtA substrates results in covalent incorporation of functional molecular handles (fluorescein, biotin, and azide) into cell wall peptidoglycan. These conclusions are supported by data obtained through a variety of experimental techniques (epifluorescence and electron microscopy, biochemical extraction, and mass spectrometry), and cell-wall-incorporated azide was exploited as a chemical handle to perform an azide-alkyne cycloaddition reaction on the bacterial cell surface. This report represents the first example of cell wall engineering of S. aureus or any other pathogenic Gram-positive bacteria and has the potential for widespread utility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.