ArticleParasitology research2011
Protein change of intestinal epithelial cells induced in vitro by Trichinella spiralis infective larvae.
Article in Parasitology research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 55 citations in OpenAlex.
- Vaccination of Mice with a Novel Trypsin fromVaccines · 2020Article
- Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity.Veterinary research · 2020Article
- Molecular characterization of a Trichinella spiralis elastase-1 and its potential as a diagnostic antigen for trichinellosis.Parasites & vectors · 2020Article
- Vaccination of mice with a recombinant novel cathepsin B inhibits Trichinella spiralis development, reduces the fecundity and worm burden.Parasites & vectors · 2019Article
- In vitro silencing of a serine protease inhibitor suppresses Trichinella spiralis invasion, development, and fecundity.Parasitology research · 2019Article
- Oral vaccination with Trichinella spiralis DNase II DNA vaccine delivered by attenuated Salmonella induces a protective immunity in BALB/c mice.Veterinary research · 2018Article
- Molecular characterization of a 31 kDa protein from Trichinella spiralis and its induced immune protection in BALB/c mice.Parasites & vectors · 2018Article
- Characterization of a serine protease inhibitor from Trichinella spiralis and its participation in larval invasion of host's intestinal epithelial cells.Parasites & vectors · 2018Article
- Molecular characterization of Trichinella spiralis galectin and its participation in larval invasion of host's intestinal epithelial cells.Veterinary research · 2018Article
- Molecular characterization of a putative serine protease from Trichinella spiralis and its elicited immune protection.Veterinary research · 2018Article
- Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool.PLoS neglected tropical diseases · 2018Article
- The Immune Protection Induced by a Serine Protease Inhibitor From the Foodborne ParasiteFrontiers in microbiology · 2018Article
- Characterization of TwoFrontiers in microbiology · 2018Article
- Helminths and intestinal barrier function.Tissue barriers · 2017Review
- Screening and characterization of early diagnostic antigens in excretory-secretory proteins from Trichinella spiralis intestinal infective larvae by immunoproteomics.Parasitology research · 2016Article
- Early serodiagnosis of trichinellosis by ELISA using excretory-secretory antigens of Trichinella spiralis adult worms.Parasites & vectors · 2015Article
- Molecular identification and characterization of Trichinella spiralis proteasome subunit beta type-7.Parasites & vectors · 2015Article
- Secretory Products of Trichinella spiralis Muscle Larvae and Immunomodulation: Implication for Autoimmune Diseases, Allergies, and Malignancies.Journal of immunology research · 2015Review
- Molecular identification of Trichinella spiralis nudix hydrolase and its induced protective immunity against trichinellosis in BALB/c mice.Parasites & vectors · 2014Article
- Molecular characterization of a Spirometra mansoni antigenic polypeptide gene encoding a 28.7 kDa protein.Parasitology research · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to observe the protein changes of intestinal epithelial cells induced in vitro by Trichinella spiralis infective larvae and their excretory-secretory (ES) or surface antigens and identity the proteins related with invasion. HCT-8 cells were incubated for 2 h in the culture medium contained ES or surface antigens of infective larvae, and observed by Immunofluorescent test (IFT). The infective larvae were inoculated into culture of HCT-8 cells to incubate for 18 h, and the lysates of HCT-8 cells were analyzed by SDS-PAGE and Western blot. IFA showed that normal HCT-8 cells had positively reactions with sera of the infected mice and mice immunized with ES or surface antigens. However, after incubating with ES or surface antigens, HCT-8 cells had stronger positively reaction with the above sera. On Western blot, after cultured with infective larvae, additional seven protein bands (66, 61, 57, 45, 34, 21, and 17 kDa) of HCT-8 cells were recognized by sera of the infected or immunized mice, but three protein bands (48, 43, and 23 kDa) of HCT-8 cells were not recognized by the above sera, compared with normal HCT-8 cells. Our results showed that after cultured with infective larvae the protein components of HCT-8 cell changed, suggesting that additional seven proteins recognized by sera of the infected or immunized mice may be related with invasion of intestinal epithelial cells by infective larvae, these proteins might mediate or facilitate entry into the cells, while the three proteins not recognized by the above sera may be the specific mediators released from the cells which permit invasion.
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