ArticleJournal of immunology (Baltimore, Md. : 1950)2010
Error-prone DNA repair activity during somatic hypermutation in shark B lymphocytes.
Article in Journal of immunology (Baltimore, Md. : 1950), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Evolutionary Comparative Analyses of DNA-Editing Enzymes of the Immune System: From 5-Dimensional Description of Protein Structures to Immunological Insights and Applications to Protein Engineering.Frontiers in immunology · 2021Pooled it
- Heavy chain-only antibody genes in fish evolved to generate unique CDR3 repertoire.European journal of immunology · 2022Article
- On Origin and Evolution of the Antibody Molecule.Biology · 2021Review
- Lost structural and functional inter-relationships between Ig and TCR loci in mammals revealed in sharks.Immunogenetics · 2021Review
- Ancient Use of Ig Variable Domains Contributes Significantly to the TCRδ Repertoire.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- JMolecular immunology · 2018Article
- Assembly and Expression of Shark Ig Genes.Journal of immunology (Baltimore, Md. : 1950) · 2016Review
- Biased Immunoglobulin Light Chain Gene Usage in the Shark.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Review
- DNA polymerase ζ generates tandem mutations in immunoglobulin variable regions.The Journal of experimental medicine · 2012Article
- Origin of immunoglobulin isotype switching.Current biology : CB · 2012Article
- The multiple shark Ig H chain genes rearrange and hypermutate autonomously.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Sharks are representatives of the earliest vertebrates that possess an immune system utilizing V(D)J recombination to generate Ag receptors. Their Ab repertoire diversity is based in part on a somatic hypermutation process that introduces adjacent nucleotide substitutions of 2-5 bp. We have isolated mutant nonfunctional Ig rearrangements and intronic flank sequences to characterize the nonselected, intrinsic properties of this phenomenon; changes unique to shark were observed. Duplications and deletions were associated with N additions, suggesting participation of a DNA polymerase with some degree of template independence during the repair of DNA breaks initiated by activation-induced cytidine deaminase. Other mutations were consistent with some in vitro activities of mammalian translesion DNA polymerase η: tandem base substitutions, strand slippage, and small insertions/deletions. The nature of substitution patterns shows that DNA lesions at shark Ig genes recruit DNA repair factors with a species-specific repertoire of activities. We speculate that the tandem mutations are introduced by direct sequential misinsertions and that, in shark B cells, the mispairs tend to be extended rather than proofread. Despite extensive changes undergone by some mutants, the physical range of mutational activity remained restricted to VDJ and within the first 2-kb portion of the 6.8-kb J-C intron, perhaps a self-regulating aspect of activation-induced cytidine deaminase action that is conserved in evolution.
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Registered trials
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