ArticleProceedings of the National Academy of Sciences of the United States of America2010
Connexin 43 is critical to maintain the homeostasis of the blood-testis barrier via its effects on tight junction reassembly.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 163 citations in OpenAlex.
- Testicular Gap (Animals : an open access journal from MDPI · 2026Article
- Microbial metabolites short chain fatty acids, tight junction, gap junction, and reproduction: a review.Frontiers in cell and developmental biology · 2025Review
- Article
- Effects of prenatal stress on reproductive function of male offspring through the KISS1 system.Endocrine connections · 2024Article
- Human coronavirus OC43 infection remodels connexin 43-mediated gap junction intercellular communicationJournal of virology · 2024Article
- Blood-testis barrier: a review on regulators in maintaining cell junction integrity between Sertoli cells.Cell and tissue research · 2024Review
- SARS-CoV-2 Structural Proteins Modulated Blood-Testis Barrier-Related Proteins through Autophagy in the Primary Sertoli Cells.Viruses · 2023Article
- Article
- Lactoferrin Restores the Deoxynivalenol-Impaired Spermatogenesis and Blood-Testis Barrier Integrity via Improving the Antioxidant Capacity and Modifying the Cell Adhesion and Inflammatory Response.Antioxidants (Basel, Switzerland) · 2023Article
- Article
- AAV-BR1 targets endothelial cells in the retina to reveal their morphological diversity and to deliver Cx43.The Journal of comparative neurology · 2022Article
- The MAPK/ERK signaling pathway regulates the expression and localization of Cx43 in mouse proximal epididymis†.Biology of reproduction · 2022Article
- Review
- COVID-19 disrupts the blood-testis barrier through the induction of inflammatory cytokines and disruption of junctional proteins.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2021Article
- Article
- Low Doses of Glyphosate/Roundup Alter Blood-Testis Barrier Integrity in Juvenile Rats.Frontiers in endocrinology · 2021Article
- Bisphenols and phthalates: Plastic chemical exposures can contribute to adverse cardiovascular health outcomes.Birth defects research · 2020Review
- Establishment and functional characterization of a murine primary Sertoli cell line deficient of connexin43.Cell and tissue research · 2020Article
- BRG1 Is Dispensable for Sertoli Cell Development and Functions in Mice.International journal of molecular sciences · 2020Article
- Exogenous Gonadotrophin Stimulation Induces Partial Maturation of Human Sertoli Cells in a Testicular Xenotransplantation Model for Fertility Preservation.Journal of clinical medicine · 2020Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
In mammalian testes, the blood-testis barrier (BTB) or Sertoli cell barrier created by specialized junctions between Sertoli cells near the basement membrane confers an immunological barrier by sequestering the events of meiotic division and postmeiotic germ cell development from the systemic circulation. The BTB is constituted by coexisting tight junctions (TJs), basal ectoplasmic specializations, desmosomes, and gap junctions. Despite being one of the tightest blood-tissue barriers, the BTB has to restructure cyclically during spermatogenesis. A recent study showed that gap junction protein connexin 43 (Cx43) and desmosome protein plakophilin-2 are working synergistically to modulate the BTB integrity by regulating the distribution of TJ-associated proteins at the Sertoli-Sertoli cell interface. However, the precise role of Cx43 in regulating the cyclical restructuring of junctions remains obscure. In this report, the calcium switch and the bisphenol A (BPA) models were used to induce junction restructuring in primary cultures of Sertoli cells isolated from rat testes that formed a TJ-permeability barrier that mimicked the BTB in vivo. The removal of calcium by EGTA perturbed the Sertoli cell tight junction barrier, but calcium repletion allowed the "resealing" of the disrupted barrier. However, a knockdown of Cx43 in Sertoli cells by RNAi significantly reduced the kinetics of TJ-barrier resealing. These observations were confirmed using the bisphenol A model in which the knockdown of Cx43 by RNAi also perturbed the TJ-barrier reassembly following BPA removal. In summary, Cx43 is crucial for TJ reassembly at the BTB during its cyclic restructuring throughout the seminiferous epithelial cycle of spermatogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.