ArticleThe Journal of biological chemistry2010
Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- ARF GTPases Function at the Golgi.Sub-cellular biochemistry · 2026Review
- Maternal immunoglobulin G affects brain development of mouse offspring.Journal of neuroinflammation · 2024Article
- STED imaging of endogenously tagged ARF GTPases reveals their distinct nanoscale localizations.The Journal of cell biology · 2023Article
- Kinetics of Arf1 inactivation regulates Golgi organisation and function in non-adherent fibroblasts.Biology open · 2023Article
- Arf5-mediated regulation of mTORC1 at the plasma membrane.Molecular biology of the cell · 2023Article
- A voyage from the ER: spatiotemporal insights into polarized protein secretion in neurons.Frontiers in cell and developmental biology · 2023Article
- Trafficking of hormones and trophic factors to secretory and extracellular vesicles: a historical perspective and new hypothesis.Extracellular vesicles and circulating nucleic acids · 2023Article
- ARF GTPases and Their Ubiquitous Role in Intracellular Trafficking Beyond the Golgi.Frontiers in cell and developmental biology · 2021Review
- Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- The priming factor CAPS1 regulates dense-core vesicle acidification by interacting with rabconnectin3β/WDR7 in neuroendocrine cells.The Journal of biological chemistry · 2019Article
- Cargo Sorting at theCells · 2019Review
- LAMP5 in presynaptic inhibitory terminals in the hindbrain and spinal cord: a role in startle response and auditory processing.Molecular brain · 2019Article
- Deletion Involving the 7q31-32 Band at the CADPS2 Gene Locus in a Patient with Autism Spectrum Disorder and Recurrent Psychotic Syndrome Triggered by Stress.Case reports in psychiatry · 2017Article
- Article
- NF-κB signaling and vesicle transport are correlated with the reactivation of the memory trace of morphine dependence.Diagnostic pathology · 2014Article
- BIG3 inhibits insulin granule biogenesis and insulin secretion.EMBO reports · 2014Article
- Arf4 is required for Mammalian development but dispensable for ciliary assembly.PLoS genetics · 2014Article
- Article
- CAPS and Munc13: CATCHRs that SNARE Vesicles.Frontiers in endocrinology · 2013Review
- Mouse models of mutations and variations in autism spectrum disorder-associated genes: mice expressing Caps2/Cadps2 copy number and alternative splicing variants.International journal of environmental research and public health · 2013Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ca(2+)-dependent activator protein for secretion (CAPS) regulates exocytosis of catecholamine- or neuropeptide-containing dense-core vesicles (DCVs) at secretion sites, such as nerve terminals. However, large amounts of CAPS protein are localized in the cell soma, and the role of somal CAPS protein remains unclear. The present study shows that somal CAPS1 plays an important role in DCV trafficking in the trans-Golgi network. The anti-CAPS1 antibody appeared to pull down membrane fractions, including many Golgi-associated proteins, such as ADP-ribosylation factor (ARF) small GTPases. Biochemical analyses of the protein-protein interaction showed that CAPS1 interacted specifically with the class II ARF4/ARF5, but not with other classes of ARFs, via the pleckstrin homology domain in a GDP-bound ARF form-specific manner. The pleckstrin homology domain of CAPS1 showed high affinity for the Golgi membrane, thereby recruiting ARF4/ARF5 to the Golgi complex. Knockdown of either CAPS1 or ARF4/ARF5 expression caused accumulation of chromogranin, a DCV marker protein, in the Golgi, thereby reducing its DCV secretion. In addition, the overexpression of CAPS1 binding-deficient ARF5 mutants induced aberrant chromogranin accumulation in the Golgi and consequently reduced its DCV secretion. These findings implicate a functional role for CAPS1 protein in the soma, a major subcellular localization site of CAPS1 in many cell types, in regulating DCV trafficking in the trans-Golgi network; this activity occurs via protein-protein interaction with ARF4/ARF5 in a GDP-dependent manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.