ArticleGene expression patterns : GEP
Distinct tissue distributions and subcellular localizations of differently phosphorylated forms of the myosin regulatory light chain in Drosophila.
Article in Gene expression patterns : GEP. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- In vivo regulation of an endogenously tagged protein by a light-regulated kinase.G3 (Bethesda, Md.) · 2025Article
- Arf1 and ARFGEF2/Sec71 control neuroblast polarity by anchoring nonmuscle myosin II.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Actomyosin contraction in the follicular epithelium provides the major mechanical force for follicle rupture duringProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Apical cell expansion maintained by Dusky-like establishes a scaffold for corneal lens morphogenesis.Science advances · 2024Article
- JAK-STAT-dependent contact between follicle cells and the oocyte controls Drosophila anterior-posterior polarity and germline development.Nature communications · 2024Article
- Medioapical contractile pulses coordinated between cells regulate Drosophila eye morphogenesis.The Journal of cell biology · 2024Article
- Cortical tension promotes Kibra degradation via Par-1.Molecular biology of the cell · 2024Article
- Engineered kinases as a tool for phosphorylation of selected targets in vivo.The Journal of cell biology · 2022Article
- The Drosophila anterior-posterior axis is polarized by asymmetric myosin activation.Current biology : CB · 2022Article
- Silencing the Myosin Regulatory Light Chain GeneInsects · 2020Article
- Sidekick Is a Key Component of Tricellular Adherens Junctions that Acts to Resolve Cell Rearrangements.Developmental cell · 2019Article
- Drak/STK17A Drives Neoplastic Glial Proliferation through Modulation of MRLC Signaling.Cancer research · 2019Article
- Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress.Developmental cell · 2019Article
- Actomyosin-Driven Tension at Compartmental Boundaries Orients Cell Division Independently of Cell Geometry In Vivo.Developmental cell · 2018Article
- Yorkie Functions at the Cell Cortex to Promote Myosin Activation in a Non-transcriptional Manner.Developmental cell · 2018Article
- Suppression of epithelial folding at actomyosin-enriched compartment boundaries downstream of Wingless signalling inDevelopment (Cambridge, England) · 2018Article
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- Spatio-temporally separated cortical flows and spindle geometry establish physical asymmetry in fly neural stem cells.Nature communications · 2017Article
- Establishment of the Muscle-Tendon Junction During Thorax Morphogenesis in Drosophila Requires the Rho-Kinase.Genetics · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nonmuscle myosin II (myosin hereafter) has well-established roles in generating contractile force on actin filaments during morphogenetic processes in all metazoans. Myosin activation is regulated by phosphorylation of the myosin regulatory light chain (MRLC, encoded by spaghettisquash or sqh in Drosophila) first on Ser21 and subsequently on Thr20. These phosphorylation events are positively controlled by a variety of kinases including myosin light chain kinase, Rho kinase, citron kinase, and AMP kinase and are negatively regulated by myosin phosphatase. The activation of myosin is thus highly regulated and likely developmentally controlled. In order to monitor the activity of myosin during development, we have generated antibodies against the monophosphorylated (Sqh1P) and diphosphorylated (Sqh2P) forms of Sqh. We first show that the antibodies are highly specific. We then used these antibodies to monitor myosin activation in wild type Drosophila tissues. Interestingly, Sqh1P and Sqh2P show distinct patterns of expression in embryos. Sqh1P is expressed nearly ubiquitously and outlines cells consistent with a junctional localization, whereas Sqh2P is strongly expressed on the apical surfaces and in filopodia of tissues undergoing extensive cell shape change or cell movements including the invaginating fore- and hindgut, the invaginating tracheal system, the dorsal pouch and the dorsal most row of epidermal (DME) cells during dorsal closure. In imaginal discs, Sqh1P predominantly localizes in the adherens junction, whereas Sqh2P locates to the apical domain. These antibodies thus have the potential to be very useful in monitoring myosin activation for functional studies of morphogenesis in Drosophila.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.