ArticleToxicology2011
Gene expression profiling of DEHP-treated cardiomyocytes reveals potential causes of phthalate arrhythmogenicity.
Article in Toxicology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Effects of Di(2-ethylhexyl) phthalate (DEHP) and hyperoxia on cardiovascular development in newborn rats.Pediatric research · 2026Article
- Common Chemical Plasticizer Di(2-Ethhylhexyl) Phthalate Exposure Exacerbates Coxsackievirus B3 Infection.Viruses · 2024Article
- Evidence for the cardiodepressive effects of the plasticizer di-2-ethylhexyl phthalate.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Granulocyte-colony stimulating factor ameliorates di-ethylhexyl phthalate-induced cardiac muscle injury via stem cells recruitment, Desmin protein regulation, antifibrotic and antiapoptotic mechanisms.Journal of molecular histology · 2023Article
- Phthalates and attributable mortality: A population-based longitudinal cohort study and cost analysis.Environmental pollution (Barking, Essex : 1987) · 2022Article
- Article
- Chronic perinatal hypoxia delays cardiac maturation in a mouse model for cyanotic congenital heart disease.American journal of physiology. Heart and circulatory physiology · 2021Article
- Bisphenols and phthalates: Plastic chemical exposures can contribute to adverse cardiovascular health outcomes.Birth defects research · 2020Review
- Phthalates Implications in the Cardiovascular System.Journal of cardiovascular development and disease · 2020Review
- TGF-β1 relieves epithelial-mesenchymal transition reduction in hypospadias induced by DEHP in rats.Pediatric research · 2020Article
- Di (2-ethylhexyl) phthalate induces cardiac disorders in BALB/c mice.Environmental science and pollution research international · 2019Article
- Does plastic chemical exposure contribute to sudden death of patients on dialysis?Heart rhythm · 2019Review
- Food Additives and Child Health.Pediatrics · 2018Article
- Food Additives and Child Health.Pediatrics · 2018Review
- Plastics and cardiovascular health: phthalates may disrupt heart rate variability and cardiovascular reactivity.American journal of physiology. Heart and circulatory physiology · 2017Article
- In Utero and Lactational Exposure Study in Rats to Identify Replacements for Di(2-ethylhexyl) Phthalate.Scientific reports · 2017Article
- The effects of environmental chemicals on renal function.Nature reviews. Nephrology · 2015Review
- Physiological response of cardiac tissue to bisphenol A: alterations in ventricular pressure and contractility.American journal of physiology. Heart and circulatory physiology · 2015Article
- Parental occupational exposures to endocrine disruptors and the risk of simple isolated congenital heart defects.Pediatric cardiology · 2015Article
- Exposure to phthalates affects calcium handling and intercellular connectivity of human stem cell-derived cardiomyocytes.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundDi-(2-ethylhexyl)-phthalate (DEHP) is a widely used plasticizer that imparts flexibility to polyvinyl chloride. We have recently reported that clinically relevant concentrations of DEHP can affect electrical coupling between cardiac myocytes causing significant rhythm disturbances. The underlying causes for this effect are currently unknown.
objectivesTo use data on global mRNA expression as a tool to reveal possible pathways leading to arrhythmogenic effects of DEHP.
methodsRat neonatal cardiomyocytes were treated with 50 μg/mL DEHP for 72 h. Extracted RNA samples were hybridized onto Affymetrix Rat Gene 1.0 ST arrays. The mRNA expression of a subset of genes was validated by qRT-PCR. In a second set of experiments, cells were treated in a concentration dependent manner to identify genes affected by low DEHP concentrations.
resultsDEHP exposure is associated with global changes in mRNA expression, with differentially expressed genes overrepresented in 47 Gene Ontology categories. Modified expression was detected for genes associated with cell electrical activity, calcium handling, adhesion and microtubular transport. For a number of key proteins, including kinesin, TGFβ2, α-tubulin, and α1 & β1 integrins, changes in mRNA levels were confirmed on the level of the protein expression. A number of genes associated with cell adhesion and electrical activity were identified as early DEHP targets as they were affected by concentrations as low as 1 μg/mL.
conclusionsExposure of neonatal rat cardiomyocytes to clinically relevant DEHP concentrations leads to global changes in mRNA expression. These changes help to explain the arrhythmogenic effects of phthalates on these cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.