ArticleBMC genomics2010
An improved, high-quality draft genome sequence of the Germination-Arrest Factor-producing Pseudomonas fluorescens WH6.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 57 citations in OpenAlex.
- Type III secretion system diversity in the Pseudomonas fluorescens species complex.The New phytologist · 2026Article
- Advances on Bioactive Metabolites with Potential for the Biocontrol of Plant Pathogenic Bacteria.Pathogens (Basel, Switzerland) · 2024Review
- Exploration of Social Spreading Reveals That This Behavior Is Prevalent amongApplied and environmental microbiology · 2021Article
- Unexpected distribution of the 4-formylaminooxyvinylglycine (FVG) biosynthetic pathway in Pseudomonas and beyond.PloS one · 2021Article
- Characterization of the SPI-1 Type III Secretion System inFrontiers in microbiology · 2021Article
- Genome Mining and Evaluation of the Biocontrol Potential ofInternational journal of molecular sciences · 2020Article
- CRISPR interference to interrogate genes that control biofilm formation in Pseudomonas fluorescens.Scientific reports · 2019Article
- Insights into the Draft Genome Sequence of the Kiwifruit-Associated Pathogenic Isolate Pseudomonas fluorescens AHK-1.Current microbiology · 2019Article
- Article
- Dynamics of Aspen Roots Colonization by Pseudomonads Reveals Strain-Specific and Mycorrhizal-Specific Patterns of Biofilm Formation.Frontiers in microbiology · 2018Article
- Article
- Genome Mining and Predictive Functional Profiling of Acidophilic RhizobacteriumIndian journal of microbiology · 2017Article
- Draft Genome Sequences of Seven 4-Formylaminooxyvinylglycine Producers Belonging to theGenome announcements · 2017Article
- Article
- Rice-Infecting Pseudomonas Genomes Are Highly Accessorized and Harbor Multiple Putative Virulence Mechanisms to Cause Sheath Brown Rot.PloS one · 2015Article
- Controlling weeds with fungi, bacteria and viruses: a review.Frontiers in plant science · 2015Review
- Article
- Microbiology, genomics, and clinical significance of the Pseudomonas fluorescens species complex, an unappreciated colonizer of humans.Clinical microbiology reviews · 2014Review
- Commonalities and differences of T3SSs in rhizobia and plant pathogenic bacteria.Frontiers in plant science · 2014Review
- Pseudomonas fluorescens NZI7 repels grazing by C. elegans, a natural predator.The ISME journal · 2013Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPseudomonas fluorescens is a genetically and physiologically diverse species of bacteria present in many habitats and in association with plants. This species of bacteria produces a large array of secondary metabolites with potential as natural products. P. fluorescens isolate WH6 produces Germination-Arrest Factor (GAF), a predicted small peptide or amino acid analog with herbicidal activity that specifically inhibits germination of seeds of graminaceous species.
resultsWe used a hybrid next-generation sequencing approach to develop a high-quality draft genome sequence for P. fluorescens WH6. We employed automated, manual, and experimental methods to further improve the draft genome sequence. From this assembly of 6.27 megabases, we predicted 5876 genes, of which 3115 were core to P. fluorescens and 1567 were unique to WH6. Comparative genomic studies of WH6 revealed high similarity in synteny and orthology of genes with P. fluorescens SBW25. A phylogenomic study also placed WH6 in the same lineage as SBW25. In a previous non-saturating mutagenesis screen we identified two genes necessary for GAF activity in WH6. Mapping of their flanking sequences revealed genes that encode a candidate anti-sigma factor and an aminotransferase. Finally, we discovered several candidate virulence and host-association mechanisms, one of which appears to be a complete type III secretion system.
conclusionsThe improved high-quality draft genome sequence of WH6 contributes towards resolving the P. fluorescens species, providing additional impetus for establishing two separate lineages in P. fluorescens. Despite the high levels of orthology and synteny to SBW25, WH6 still had a substantial number of unique genes and represents another source for the discovery of genes with implications in affecting plant growth and health. Two genes are demonstrably necessary for GAF and further characterization of their proteins is important for developing natural products as control measure against grassy weeds. Finally, WH6 is the first isolate of P. fluorescens reported to encode a complete T3SS. This gives us the opportunity to explore the role of what has traditionally been thought of as a virulence mechanism for non-pathogenic interactions with plants.
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