ArticleBMC genomics2010
Identification and characterization of microRNAs in Clonorchis sinensis of human health significance.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 76 citations in OpenAlex.
- Roles of microRNAs and Long Non-Coding RNAs Encoded by Parasitic Helminths in Human Carcinogenesis.International journal of molecular sciences · 2022Review
- MicroRNA-497 induced by Clonorchis sinensis enhances the TGF-β/Smad signaling pathway to promote hepatic fibrosis by targeting Smad7.Parasites & vectors · 2021Article
- MicroRNA Interference in Hepatic Host-Pathogen Interactions.International journal of molecular sciences · 2021Review
- Computational prediction and characterisation of miRNAs and their pathway genes in human schistosomiasis caused by Schistosoma haematobium.Memorias do Instituto Oswaldo Cruz · 2020Article
- Micromanagement of Immune System: Role of miRNAs in Helminthic Infections.Frontiers in microbiology · 2017Review
- Micropathogen Community Analysis inFrontiers in cellular and infection microbiology · 2017Article
- Dysregulation of hepatic microRNA expression profiles with Clonorchis sinensis infection.BMC infectious diseases · 2016Article
- Current status and perspectives of Clonorchis sinensis and clonorchiasis: epidemiology, pathogenesis, omics, prevention and control.Infectious diseases of poverty · 2016Review
- Identification and characterization of microRNAs in the zoonotic fluke Fasciolopsis buski.Parasitology research · 2016Article
- Identification and Characterization of Erysiphe necator-Responsive MicroRNAs in Chinese Wild Vitis pseudoreticulata by High-Throughput Sequencing.Frontiers in plant science · 2016Article
- Identification and characterization of microRNAs at different flowering developmental stages in moso bamboo (Phyllostachys edulis) by high-throughput sequencing.Molecular genetics and genomics : MGG · 2015Article
- Identification of microRNA genes in three opisthorchiids.PLoS neglected tropical diseases · 2015Article
- Identification of microRNAs and target genes in the fruit and shoot tip of Lycium chinense: a traditional Chinese medicinal plant.PloS one · 2015Article
- Identification and characterization of cold-responsive microRNAs in tea plant (Camellia sinensis) and their targets using high-throughput sequencing and degradome analysis.BMC plant biology · 2014Article
- microRNAs of parasitic helminths - Identification, characterization and potential as drug targets.International journal for parasitology. Drugs and drug resistance · 2014Review
- MicroRNA expression profile in the third- and fourth-stage larvae of Angiostrongylus cantonensis.Parasitology research · 2014Article
- Comparative analysis of microRNA profiles between adult Ascaris lumbricoides and Ascaris suum.BMC veterinary research · 2014Article
- Global characterization of microRNAs in Trichomonas gallinae.Parasites & vectors · 2014Article
- Characterization and expression analysis of microRNAs in the tube foot of sea cucumber Apostichopus japonicus.PloS one · 2014Article
- Substantial loss of conserved and gain of novel MicroRNA families in flatworms.Molecular biology and evolution · 2013Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClonorchis sinensis is a zoonotic parasite causing clonorchiasis-associated human disease such as biliary calculi, cholecystitis, liver cirrhosis, and it is currently classified as carcinogenic to humans for cholangiocarcinoma. MicroRNAs (miRNAs) are non-coding, regulating small RNA molecules which are essential for the complex life cycles of parasites and are involved in parasitic infections. To identify and characterize miRNAs expressed in adult C. sinensis residing chronically in the biliary tract, we developed an integrative approach combining deep sequencing and bioinformatic predictions with stem-loop real-time PCR analysis.
resultsHere we report the use of this approach to identify and clone 6 new and 62,512 conserved C. sinensis miRNAs which belonged to 284 families. There was strong bias on families, family members and sequence nucleotides in C. sinensis. Uracil was the dominant nucleotide, particularly at positions 1, 14 and 22, which were located approximately at the beginning, middle and end of conserved miRNAs. There was no significant "seed region" at the first and ninth positions which were commonly found in human, animals and plants. Categorization of conserved miRNAs indicated that miRNAs of C. sinensis were still innovated and concentrated along three branches of the phylogenetic tree leading to bilaterians, insects and coelomates. There were two miRNA strategies in C. sinensis for its parasitic life: keeping a large category of miRNA families of different animals and keeping stringent conserved seed regions with high active innovation in other places of miRNAs mainly in the middle and the end, which were perfect for the parasite to perform its complex life style and for host changes.
conclusionsThe present study represented the first large scale characterization of C. sinensis miRNAs, which have implications for understanding the complex biology of this zoonotic parasite, as well as miRNA studies of other related species such as Opisthorchis viverrini and Opisthorchis felineus of human and animal health significance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.