Trial reportAmerican journal of veterinary research2010
Comparison of iatrogenic transmission of Anaplasma marginale in Holstein steers via needle and needle-free injection techniques.
Trial report in American journal of veterinary research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Molecular Detection of Anaplasma marginale in the Central Region of Venezuela: A PCR-based Assessment of the msp2 and msp5 Genes.Acta parasitologica · 2026Article
- Bacillary hemoglobinuria in a dairy cow in Goiás State, Central-West Brazil, without demonstrable evidence of active fascioliasis.Veterinary research communications · 2026Article
- Vertical transmission, infection dynamics and risk factors for the occurrence of tick fever agents in beef cattle from a tropical region.Tropical animal health and production · 2026Article
- Serological Assessment of Enzootic Status and Associated Risk Factors for Cattle Tick Fever in Herds from Paraíba State, Northeast Brazil.Pathogens (Basel, Switzerland) · 2026Article
- Demonstration of Anaplasma marginale transmission to cattle through the intra-transstadial and by unfed larvae of Rhipicephalus microplus routes, with subsequent calculation of infection rates.Tropical animal health and production · 2026Article
- Molecular Prevalence of Hemotropic Mycoplasmosis and Associated Risk Factors for Co-Infection with Gastrointestinal Nematode in Anemic Meat Goats of Northeast Thailand.Animals : an open access journal from MDPI · 2026Article
- Tick fever risk and factors influencing the transmission of Anaplasma marginale, Babesia bovis and Babesia bigemina in cattle properties from Atlantic Forest biome.Tropical animal health and production · 2026Article
- Epidemiological Spectrum of Bovine Tick-Borne Pathogens in Northeast Brazil: Comparative Analysis Across a Tropical Humid and Two Semi-Arid Regions.Pathogens (Basel, Switzerland) · 2025Article
- Needle-free injection device for administration of cloprostenol to induce luteolysis in lactating dairy cows.JDS communications · 2025Article
- Leveraging metatranscriptomics for the characterisation of bovine blood viromes.Scientific reports · 2025Article
- Seroprevalence and Risk Factors of Anaplasma marginale in Water Buffaloes in Nile Delta of Egypt.Acta parasitologica · 2025Article
- Article
- Bayesian spatiotemporal evaluation of bovine anaplasmosis seroprevalence in Missouri (2010-2021).Frontiers in veterinary science · 2025Article
- A Cross-Sectional Serological Study to Assess the Prevalence and Risk Factors of Anaplasmosis in Dromedary Camels in Punjab, Pakistan.Veterinary sciences · 2024Article
- First Report ofPathogens (Basel, Switzerland) · 2024Article
- Anaplasmosis outbreaks in taurine and zebu cattle in Brazil: causes, treatments, and Anaplasma marginale genetic diversity.Tropical animal health and production · 2024Article
- Enzootic stability of tick fever in Holstein calves grazing in a tropical region, subjected to strategic cattle tick control with fluralaner.Parasites & vectors · 2024Article
- A novel needle-free method of lidocaine administration during standing castration of Holstein bulls.JDS communications · 2024Article
- Immunization withVaccines · 2023Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
objectiveTo compare iatrogenic transmission of Anaplasma marginale during sham vaccination between needle and needle-free injection techniques. ANIMALS: 26 Holstein steers confirmed negative for anaplasmosis by use of a competitive ELISA (cELISA) and an A marginale-specific reverse transcription (RT)-PCR assay. PROCEDURES: An isolate of A marginale was propagated to a circulating parasitemia of 2.0% in a splenectomized steer. Sham vaccination was performed in the left cervical muscles of the splenectomized parasitemic steer with a hypodermic needle fitted to a multiple-dose syringe. The same needle and syringe were used to sham vaccinate a naïve steer. This 2-step procedure was repeated until 10 naïve steers (group ND) were injected. Similarly, sham vaccination of the left cervical muscles of the splenectomized parasitemic steer and another group of 10 naïve steers (group NF) was performed by use of a needle-free injection system. Five control steers were not injected. Disease status was evaluated twice weekly for 61 days by use of light microscopy, a cELISA, and an A marginale-specific RT-PCR assay.
resultsIatrogenic transmission was detected in 6 of 10 steers in group ND. Disease status did not change in the NF or control steers. Sensitivity of light microscopy, cELISA, and RT-PCR assay was 100% on days 41, 41, and 20 after sham vaccination, respectively; however, only cELISA and RT-PCR assay sustained a sensitivity of 100% thereafter. CONCLUSIONS AND CLINICAL RELEVANCE: Needle-free injection was superior to needle injection for the control of iatrogenic transmission of A marginale.
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