ArticleNature biotechnology2010
Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage.
Article in Nature biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 5 registered trials, which are not on this map. Cited by 275 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Eeva™ System Study: Noninvasive Recording and Visualization of Individual Embryos Cultured to Blastocyst Stage
Time-Lapse Incubation for Embryo Culture - The Impact of Morphokinetics and Environmental Stability on Reproductive Outcomes
Cell-free DNA Analysis of Spent Embryo Culture Media as a Non-invasive Approach for Preimplantation Genetic Diagnosis
An Observational Study of Artificial Intelligence-Assisted Blastocyst Morphokinetic Analysis Using Short-Duration Video and Its Association With Pregnancy Outcomes
Assessing the Correlation Between Phospholipase C Zeta (PLCZeta) Measurements and Semen Parameters
Who cites it
275 citing papers in PubMed, 3 syntheses or guidelines pooled it, 784 citations in OpenAlex.
- The impact of post thaw embryo culture (extended culture) on frozen embryo transfer outcomes: a systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2026Pooled it
- Molecular Drivers of Developmental Arrest in the Human Preimplantation Embryo: A Systematic Review and Critical Analysis Leading to Mapping Future Research.International journal of molecular sciences · 2021Pooled it
- Pooled it
- Trial
- Impact of Maturation and Vitrification Time of Human GV Oocytes on the Metaphase Plate Configuration.International journal of molecular sciences · 2021Trial
- Different effectiveness of closed embryo culture system with time-lapse imaging (EmbryoScope(TM)) in comparison to standard manual embryology in good and poor prognosis patients: a prospectively randomized pilot study.Reproductive biology and endocrinology : RB&E · 2016Trial
- Addressing multiple pregnancies in in vitro fertilization-embryo transfer: Current status, challenges, and solutions.International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics · 2026Review
- PGT-A reveals triploid embryos among normally fertilized oocytes: frequency and blastocyst features.Journal of assisted reproduction and genetics · 2026Article
- Cumulus cell DNA fragmentation in relation to oocyte yield and blastocyst quality.Reproduction & fertility · 2026Article
- Spatial and Molecular Parameters of Pre-OPU Ovarian Follicles and Their Association with Embryo Developmental Competence in Assisted Reproductive Technology.International journal of molecular sciences · 2026Article
- Applications of artificial intelligence in bovine reproductive assessment: focus on oocytes and blastocysts.Journal of assisted reproduction and genetics · 2026Review
- Genetic Mutations and Non-Genomic Dysregulation in Human Preimplantation Embryo Arrest.International journal of molecular sciences · 2026Review
- MiMics-Net: A Multimodal Interaction Network for Blastocyst Component Segmentation.Diagnostics (Basel, Switzerland) · 2026Article
- AI prediction models based on time-lapse imaging for good embryos with implantation potential and euploidy.Scientific reports · 2026Article
- Polycystic ovary syndrome (PCOS) affects relative embryo morphokinetics observed by time-lapse imaging: an observational study.Archives of gynecology and obstetrics · 2026Observational
- Non-invasive assessment of embryo development and ploidy: morphokinetics predict blastulation, and exploratory metabolomics identifies candidate metabolic signatures for euploidy.Frontiers in cell and developmental biology · 2026Article
- Morphokinetic parameter S2 predicts early embryo developmental potential and associated metabolic pathways.Journal of assisted reproduction and genetics · 2026Article
- Collagen nanofiber reinforced alginate hydrogel tube microbioreactors for cell culture.Frontiers in bioengineering and biotechnology · 2026Article
- Nuclear Dynamics and Its Timing Regulation Revealed by Live-Cell Imaging.Advances in experimental medicine and biology · 2026Review
- Spindle-to-oocyte light retardance ratio as a noninvasive biomarker for oocyte quality assessment: a prospective cohort study.Frontiers in endocrinology · 2026Article
215 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report studies of preimplantation human embryo development that correlate time-lapse image analysis and gene expression profiling. By examining a large set of zygotes from in vitro fertilization (IVF), we find that success in progression to the blastocyst stage can be predicted with >93% sensitivity and specificity by measuring three dynamic, noninvasive imaging parameters by day 2 after fertilization, before embryonic genome activation (EGA). These parameters can be reliably monitored by automated image analysis, confirming that successful development follows a set of carefully orchestrated and predictable events. Moreover, we show that imaging phenotypes reflect molecular programs of the embryo and of individual blastomeres. Single-cell gene expression analysis reveals that blastomeres develop cell autonomously, with some cells advancing to EGA and others arresting. These studies indicate that success and failure in human embryo development is largely determined before EGA. Our methods and algorithms may provide an approach for early diagnosis of embryo potential in assisted reproduction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.