ArticleApplied and environmental microbiology2010
Increased ethanol productivity in xylose-utilizing Saccharomyces cerevisiae via a randomly mutagenized xylose reductase.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 94 citations in OpenAlex.
- Regulatory mechanism of Haa1p and Hap4p in Saccharomyces cerevisiae to mixed acetic acid and formic acid when fermenting mixed glucose and xylose.Microbial cell factories · 2025Article
- Metabolic engineering of Saccharomyces cerevisiae for efficient utilization of pectin-rich biomass.Journal of microbiology (Seoul, Korea) · 2025Review
- Review
- Review
- Different transcriptional responses of haploid and diploid S. cerevisiae strains to changes in cofactor preference of XR.Microbial cell factories · 2020Article
- Rewired cellular signaling coordinates sugar and hypoxic responses for anaerobic xylose fermentation in yeast.PLoS genetics · 2019Article
- Xylose utilization stimulates mitochondrial production of isobutanol and 2-methyl-1-butanol inBiotechnology for biofuels · 2019Article
- Structural insight intoScientific reports · 2018Article
- Genomic and phenotypic characterization of a refactored xylose-utilizingBiotechnology for biofuels · 2018Article
- The amino-terminal tail of Hxt11 confers membrane stability to the Hxt2 sugar transporter and improves xylose fermentation in the presence of acetic acid.Biotechnology and bioengineering · 2017Article
- Saccharomyces cerevisiae strains for second-generation ethanol production: from academic exploration to industrial implementation.FEMS yeast research · 2017Review
- Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective.Microbial cell factories · 2017Review
- Mutations in PMR1 stimulate xylose isomerase activity and anaerobic growth on xylose of engineered Saccharomyces cerevisiae by influencing manganese homeostasis.Scientific reports · 2017Article
- Enhanced xylose fermentation by engineered yeast expressing NADH oxidase through high cell density inoculums.Journal of industrial microbiology & biotechnology · 2017Article
- Close to the Edge: Growth Restrained by the NAD(P)H/ATP Formation Flux Ratio.Frontiers in microbiology · 2017Article
- Anaerobic poly-3-D-hydroxybutyrate production from xylose in recombinant Saccharomyces cerevisiae using a NADH-dependent acetoacetyl-CoA reductase.Microbial cell factories · 2016Article
- Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae.Biotechnology for biofuels · 2016Article
- Review
- Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose.AMB Express · 2015Article
- Improving industrial yeast strains: exploiting natural and artificial diversity.FEMS microbiology reviews · 2014Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Baker's yeast (Saccharomyces cerevisiae) has been genetically engineered to ferment the pentose sugar xylose present in lignocellulose biomass. One of the reactions controlling the rate of xylose utilization is catalyzed by xylose reductase (XR). In particular, the cofactor specificity of XR is not optimized with respect to the downstream pathway, and the reaction rate is insufficient for high xylose utilization in S. cerevisiae. The current study describes a novel approach to improve XR for ethanol production in S. cerevisiae. The cofactor binding region of XR was mutated by error-prone PCR, and the resulting library was expressed in S. cerevisiae. The S. cerevisiae library expressing the mutant XR was selected in sequential anaerobic batch cultivation. At the end of the selection process, a strain (TMB 3420) harboring the XR mutations N272D and P275Q was enriched from the library. The V(max) of the mutated enzyme was increased by an order of magnitude compared to that of the native enzyme, and the NADH/NADPH utilization ratio was increased significantly. The ethanol productivity from xylose in TMB 3420 was increased ∼40 times compared to that of the parent strain (0.32 g/g [dry weight {DW}] × h versus 0.007 g/g [DW] × h), and the anaerobic growth rate was increased from ∼0 h(-1) to 0.08 h(-1). The improved traits of TMB 3420 were readily transferred to the parent strain by reverse engineering of the mutated XR gene. Since integrative vectors were employed in the construction of the library, transfer of the improved phenotype does not require multicopy expression from episomal plasmids.
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