ArticleHuman reproduction (Oxford, England)2010
Effect of ICSI on gene expression and development of mouse preimplantation embryos.
Article in Human reproduction (Oxford, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 72 citations in OpenAlex.
- The Fallopian tube: Biosensor and dynamic regulator of preimplantation development.PNAS nexus · 2026Review
- When less really is more: reducing embryo manipulation to lower metabolic stress and improving long term outcomes.Reproduction, fertility, and development · 2026Review
- Variable partition of non-homogeneous ooplasm sets the stage for divergent potency of 2-cell stage blastomeres.Molecular human reproduction · 2026Article
- Proteomic and metabolomic insights into oxidative stress response activation in mouse embryos generated byHuman reproduction open · 2025Article
- Long-Term Effects of ART on the Health of the Offspring.International journal of molecular sciences · 2023Review
- Fertility treatment increases the risk of preterm birth independent of multiple gestations.F&S reports · 2023Article
- Study protocol for a Developmental Epidemiological Study of Children born through Reproductive Technologies (DESCRT).Human reproduction open · 2023Review
- First-line Evaluation of Sperm Parameters in Mice (Bio-protocol · 2022Article
- Glutamate can act as a signaling molecule in mouse preimplantation embryos†.Biology of reproduction · 2022Article
- Microinjection manipulation decreases the expression of GABA-A receptor signaling pathway genes in mouse embryos derived using intracytoplasmic sperm injection.Journal of clinical laboratory analysis · 2021Article
- Transient Changes of Metabolism at the Pronuclear Stage in Mice Influences Skeletal Muscle Phenotype in Adulthood.International journal of molecular sciences · 2020Article
- Loss of methylation of H19-imprinted gene derived from assisted reproductive technologies can be mitigated by cleavage-stage embryo transfer in mice.Journal of assisted reproduction and genetics · 2019Article
- From Embryos to Adults: A DOHaD Perspective on In Vitro Fertilization and Other Assisted Reproductive Technologies.Healthcare (Basel, Switzerland) · 2016Article
- In vitro fertilization (IVF) in mammals: epigenetic and developmental alterations. Scientific and bioethical implications for IVF in humans.Biological research · 2015Review
- Genes and Conditions Controlling Mammalian Pre- and Post-implantation Embryo Development.Current genomics · 2015Article
- Article
- Some reflections on intracytoplasmic morphologically selected sperm injection.International journal of fertility & sterility · 2014Review
- Isoform-specific and protein kinase C-mediated regulation of CTP:phosphoethanolamine cytidylyltransferase phosphorylation.The Journal of biological chemistry · 2014Article
- ART and health: clinical outcomes and insights on molecular mechanisms from rodent studies.Molecular human reproduction · 2013Review
- Regulation of Phosphatidylethanolamine Homeostasis—The Critical Role of CTP:Phosphoethanolamine Cytidylyltransferase (Pcyt2).International journal of molecular sciences · 2013Article
Corrections and comments
- Erratum issuedDe Sebastiano, F [corrected to Di Sebastiano, F]
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundIn vitro culture (IVC) and IVF of preimplantation mouse embryos are associated with changes in gene expression. It is however not known whether ICSI has additional effects on the transcriptome of mouse blastocysts.
methodsWe compared gene expression and development of mouse blastocysts produced by ICSI and cultured in Whitten's medium (ICSI(WM)) or KSOM medium with amino acids (ICSI(KSOMaa)) with control blastocysts flushed out of the uterus on post coital Day 3.5 (in vivo). In addition, we compared gene expression in embryos generated by IVF or ICSI using WM. Global pattern of gene expression was assessed using the Affymetrix 430 2.0 chip.
resultsBlastocysts from ICSI fertilization have a reduction in the number of trophoblastic and inner cell mass cells compared with embryos generated in vivo. Approximately 1000 genes are differentially expressed between ICSI blastocyst and in vivo blastocysts; proliferation, apoptosis and morphogenetic pathways are the most common pathways altered after IVC. Unexpectedly, expression of only 41 genes was significantly different between embryo cultured in suboptimal conditions (WM) or optimal conditions (KSOM(aa)).
conclusionsOur results suggest that fertilization by ICSI may play a more important role in shaping the transcriptome of the developing mouse embryo than the culture media used.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.