ArticleMethods in enzymology2010
Site-specific biomolecule labeling with gold clusters.
Article in Methods in enzymology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Cloneable contrast across all biological length scales.Journal of structural biology · 2026Article
- Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- E+: Software for Hierarchical Modeling of Electron Scattering from Complex Structures.Journal of chemical information and modeling · 2025Article
- Visualizing Heterogeneous Protein Conformations with Multi-Tilt Nanoparticle-Aided Cryo-Electron Microscopy Sampling.Nano letters · 2023Article
- CryomicroscopyFaraday discussions · 2022Article
- Gold labelling of a green fluorescent protein (GFP)-tag inside cells using recombinant nanobodies conjugated to 2.4 nm thiolate-coated gold nanoparticles.Nanoscale advances · 2021Article
- The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells.Nature communications · 2021Article
- The AuMolecules (Basel, Switzerland) · 2021Article
- Site-specific covalent labeling of large RNAs with nanoparticles empowered by expanded genetic alphabet transcription.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Highly Sensitive Immunochromatographic Strip Test for Rapid and Quantitative Detection of Saikosaponin d.Molecules (Basel, Switzerland) · 2018Article
- Structural characterization of site-modified nanocapsid with monodispersed gold clusters.Scientific reports · 2017Article
- Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Surface modulatable nanocapsids for targeting and tracking toward nanotheranostic delivery.Pharmaceutical patent analyst · 2016Review
- Use of single chain antibody derivatives for targeted drug delivery.Molecular medicine (Cambridge, Mass.) · 2016Article
- Single-particle cryo-electron microscopy of macromolecular complexes.Microscopy (Oxford, England) · 2016Review
- Aspheric Solute Ions Modulate Gold Nanoparticle Interactions in an Aqueous Solution: An Optimal Way To Reversibly Concentrate Functionalized Nanoparticles.The journal of physical chemistry. B · 2015Article
- Progress toward clonable inorganic nanoparticles.Nanoscale · 2015Article
- A Note on the use of Steady-State Fluorescence Quenching to Quantify Nanoparticle-Protein Interactions.Journal of fluorescence · 2015Article
- Site-specific labeling of proteins for electron microscopy.Journal of structural biology · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Site-specific labeling of biomolecules in vitro with gold clusters can enhance the information content of electron cryomicroscopy experiments. This chapter provides a practical overview of well-established techniques for forming biomolecule/gold cluster conjugates. Three bioconjugation chemistries are covered: linker-mediated bioconjugation, direct gold-biomolecule bonding, and coordination-mediated bonding of nickel(II) nitrilotriacetic acid (NTA)-derivatized gold clusters to polyhistidine (His)-tagged proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.