ArticleACS chemical biology2011
Recombinant E. coli prototype strains for in vivo glycorandomization.
Article in ACS chemical biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Expanding Steroid Glycodiversity: Tandem Steroid Glucosylation and Acetylation via Enzymatic Cascade.International journal of molecular sciences · 2026Article
- Dysregulation of a nucleotidyltransferase induces division and surface glycan defects inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Review
- Expanding the Substrate Scope of a Bacterial Nucleotidyltransferase via Allosteric Mutations.ACS infectious diseases · 2022Article
- Article
- Exploring the broad nucleotide triphosphate and sugar-1-phosphate specificity of thymidylyltransferase Cps23FL fromRSC advances · 2020Article
- Sugar-Pirating as an Enabling Platform for the Synthesis of 4,6-Dideoxyhexoses.Journal of the American Chemical Society · 2020Article
- OleD Loki as a Catalyst for Hydroxamate Glycosylation.Chembiochem : a European journal of chemical biology · 2020Article
- Microbial production of astilbin, a bioactive rhamnosylated flavanonol, from taxifolin.World journal of microbiology & biotechnology · 2017Article
- Identification of Neuroprotective Spoxazomicin and Oxachelin Glycosides via Chemoenzymatic Glycosyl-Scanning.Journal of natural products · 2017Article
- Biosynthesis of three N-acetylaminosugar-conjugated flavonoids using engineered Escherichia coli.Microbial cell factories · 2016Article
- Glycosyltransferase-Mediated Exchange of Rare Microbial Sugars with Natural Products.Frontiers in microbiology · 2016Article
- Accessing Nature's diversity through metabolic engineering and synthetic biology.F1000Research · 2016Review
- A comprehensive review of glycosylated bacterial natural products.Chemical Society reviews · 2015Review
- Modification of emodin and aloe-emodin by glycosylation in engineered Escherihia coli.World journal of microbiology & biotechnology · 2015Article
- Enzymatic Biosynthesis of Novel Resveratrol Glucoside and Glycoside Derivatives.Applied and environmental microbiology · 2014Article
- A simple strategy for glycosyltransferase-catalyzed aminosugar nucleotide synthesis.Chembiochem : a European journal of chemical biology · 2014Article
- Glucosylation of isoflavonoids in engineered Escherichia coli.Molecules and cells · 2014Article
- Recent biotechnological progress in enzymatic synthesis of glycosides.Journal of industrial microbiology & biotechnology · 2013Review
Corrections and comments
- Commented on by
Authors and funding
4 authors.
Funding
Abstract
In vitro glycorandomization is a powerful strategy to alter the glycosylation patterns of natural products and small molecule therapeutics. Yet, such in vitro methods are often difficult to scale and can be costly given the requirement to provide various nucleotides and cofactors. Here, we report the construction of several recombinant E. coli prototype strains that allow the facile production of a range of small molecule glycosides. This strategy relies on the engineered promiscuity of three key enzymes, an anomeric kinase, a sugar-1-phosphate nucleotidyltransferase, and a glycosyltransferase, as well as the ability of diverse small molecules to freely enter E. coli. Subsequently, this work is the first demonstration of "in vivo glycorandomization" and offers vast combinatorial potential by simple fermentation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.