ArticleAnalytical chemistry2010
Phage anti-immunocomplex assay for clomazone: two-site recognition increasing assay specificity and facilitating adaptation into an on-site format.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- Development of competitive and noncompetitive lateral flow immunoassays for pendimethalin using synthetic peptides.Mikrochimica acta · 2023Article
- Small Peptides in the Detection of Mycotoxins and Their Potential Applications in Mycotoxin Removal.Toxins · 2022Review
- Identification and Application of Two Promising Peptide Ligands for the Immunodetection of Imidacloprid Residue.Foods (Basel, Switzerland) · 2022Article
- Development of a sensitive non-competitive immunoassay via immunocomplex binding peptide for the determination of ethyl carbamate in wine samples.Journal of hazardous materials · 2021Article
- Fluorescent "Turn off-on" Small-Molecule-Monitoring Nanoplatform Based on Dendrimer-like Peptides as Competitors.ACS applied materials & interfaces · 2019Article
- Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin.Analytica chimica acta · 2015Article
- Recombinant streptavidin nanopeptamer anti-immunocomplex assay for noncompetitive detection of small analytes.Analytical chemistry · 2014Article
- Enhanced sensitive immunoassay: noncompetitive phage anti-immune complex assay for the determination of malachite green and leucomalachite green.Journal of agricultural and food chemistry · 2014Article
- Virus outbreaks in chemical and biological sensors.Sensors (Basel, Switzerland) · 2014Review
- Shiga-like toxin B subunit of Escherichia coli as scaffold for high-avidity display of anti-immunocomplex peptides.Analytical chemistry · 2014Article
- Nanopeptamers for the development of small-analyte lateral flow tests with a positive readout.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The impact of the use of herbicides in agriculture can be minimized by compliance with good management practices that reduce the amount used and their release into the environment. Simple tests that provide real time on-site information about these chemicals are a major aid for these programs. In this work, we show that phage anti-immunocomplex assay (PHAIA), a method that uses phage-borne peptides to detect the formation of antibody-analyte immunocomplexes, is an advantageous technology to produce such field tests. A monoclonal antibody to the herbicide clomazone was raised and used in the development of conventional competitive and noncompetitive PHAIA immunoassays. The sensitivity attained with the PHAIA format was over 10 times higher than that of the competitive format. The cross-reactivity of the two methods was also compared using structurally related compounds, and we observed that the two-site binding of PHAIA "double-checks" the recognition of the analyte, thereby increasing the assay specificity. The positive readout of the noncompetitive PHAIA method allowed adaptation of the assay into a rapid and simple format where as little as 0.4 ng/mL clomazone (more than 10-fold lower than the proposed standard) in water samples from a rice field could be easily detected by simple visual inspection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.