ArticleDiabetologia2010
mRNA expression analysis of cell cycle genes in islets of pregnant mice.
Article in Diabetologia, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- A new paradigm of islet adaptations in human pregnancy: insights from immunohistochemistry and proteomics.Nature communications · 2025Article
- Pancreatic islet cell type-specific transcriptomic changes during pregnancy and postpartum.iScience · 2023Article
- Normal Pregnancy-Induced Islet Beta Cell Proliferation in Mouse Models That Are Deficient in Serotonin-Signaling.International journal of molecular sciences · 2022Article
- Intercellular Communication in the Islet of Langerhans in Health and Disease.Comprehensive Physiology · 2021Article
- Continuous glucose monitoring during pregnancy in healthy mice.Scientific reports · 2021Article
- FIGNL1 promotes non‑small cell lung cancer cell proliferation.International journal of oncology · 2021Observational
- Generation and Characterization of a Novel Mouse Model That Allows Spatiotemporal Quantification of Pancreatic β-Cell Proliferation.Diabetes · 2020Article
- In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals.Cell discovery · 2020Article
- Role and Significance of Circulating Biomarkers: miRNA and E2F1 mRNA Expression and Their Association with Type-2 Diabetic Complications.International journal of endocrinology · 2020Article
- Maternal β-Cell Adaptations in Pregnancy and Placental Signalling: Implications for Gestational Diabetes.International journal of molecular sciences · 2018Review
- Expression Data Analysis for the Identification of Potential Biomarker of Pregnancy Associated Breast Cancer.Pathology oncology research : POR · 2017Article
- How stable is repression of disallowed genes in pancreatic islets in response to metabolic stress?PloS one · 2017Article
- Circulating microRNA (miRNA) Expression Profiling in Plasma of Patients with Gestational Diabetes Mellitus Reveals Upregulation of miRNA miR-330-3p.Frontiers in endocrinology · 2017Article
- Advances in β cell replacement and regeneration strategies for treating diabetes.The Journal of clinical investigation · 2016Review
- Serotonin competence of mouse beta cells during pregnancy.Diabetologia · 2016Review
- Beta cell adaptation in pregnancy: a tribute to Claes Hellerström.Upsala journal of medical sciences · 2016Review
- Research Resource: A Dual Proteomic Approach Identifies Regulated Islet Proteins During β-Cell Mass Expansion In Vivo.Molecular endocrinology (Baltimore, Md.) · 2016Article
- TGF-β signalling prevents pancreatic beta cell death after proliferation.Cell proliferation · 2015Article
- Impaired proliferation of pancreatic beta cells, by reduced placental growth factor in pre-eclampsia, as a cause for gestational diabetes mellitus.Cell proliferation · 2015Article
- Prolactin receptors and placental lactogen drive male mouse pancreatic islets to pregnancy-related mRNA changes.PloS one · 2015Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisPregnancy requires an increase in the functional beta cell mass to match metabolic needs for insulin. To understand this adaptation at the molecular level, we undertook a time course analysis of mRNA expression in mice.
methodsTotal RNA extracted from C57Bl6/J mouse islets every 3 days during pregnancy was hybridised on commercially available expression arrays. Gene network analysis was performed and changes in functional clusters over time visualised. The function of putative novel cell cycle genes was assessed via silencing in replicating mouse insulinoma 6 (MIN6) cells.
resultsGene network analysis identified a large gene cluster associated with cell cycle control (67 genes, all upregulated by ≥ 1.5-fold, p < 0.001). The number of upregulated cell cycle genes and the mRNA expression levels of individual genes peaked at pregnancy day (P)9.5. Filtering of poorly annotated genes with enhanced expression in islets at P9.5, and in MIN6 cells and thymus resulted in further studies with G7e (also known as D17H6S56E-5) and Fignl1. Gene knock-down experiments in MIN6 cells suggested that these genes are indeed involved in adequate cell cycle accomplishment. CONCLUSIONS/
interpretationA sharp peak of cell cycle-related mRNA expression in islets occurs around P9.5, after which beta cell replication is increased. As illustrated by the identification of G7e and Fignl1 in islets of pregnant mice, further study of this distinct transcriptional peak should help to unravel the complex process of beta cell replication.
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