ArticleChemical engineering science2010
Utilizing Simple Biochemical Measurements to Predict Lifetime Output of Biocatalysts in Continuous Isothermal Processes.
Article in Chemical engineering science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Directed Evolution of a Modular Polyketide Synthase Thioesterase for Generation of a Hybrid Macrocyclic Ring System.ACS catalysis · 2025Article
- Molecular Engineering L-Aspartate-Alpha-Decarboxylase to Enhance Catalytic Stability and Performance.ChemistryOpen · 2025Article
- Purification and characterization of a Rieske oxygenase and its NADH-regenerating partner proteins.Methods in enzymology · 2024Article
- Improvement of α-amino Ester Hydrolase Stability via Computational Protein Design.The protein journal · 2023Article
- A selective and atom-economic rearrangement of uridine by cascade biocatalysis for production of pseudouridine.Nature communications · 2023Article
- Mutations Increasing Cofactor Affinity, Improve Stability and Activity of a Baeyer-Villiger Monooxygenase.ACS catalysis · 2022Article
- Biochemical and structural characterization of an aromatic ring-hydroxylating dioxygenase for terephthalic acid catabolism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- An Engineered Cytidine Deaminase for Biocatalytic Production of a Key Intermediate of the Covid-19 Antiviral Molnupiravir.Journal of the American Chemical Society · 2022Article
- The Moderately (D)efficient Enzyme: Catalysis-Related DamageBiochemistry · 2021Article
- Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering.Nature communications · 2021Article
- Article
- E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene-reductases*.Angewandte Chemie (International ed. in English) · 2021Article
- The number of catalytic cycles in an enzyme's lifetime and why it matters to metabolic engineering.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Improving the Thermostability ofFrontiers in microbiology · 2020Article
- Considerations when Measuring Biocatalyst Performance.Molecules (Basel, Switzerland) · 2019Review
- Ionic liquids and protein folding-old tricks for new solvents.Biophysical reviews · 2019Review
- Terminal Alkenes from Acrylic Acid Derivatives via Non-Oxidative Enzymatic Decarboxylation by Ferulic Acid Decarboxylases.ChemCatChem · 2018Article
- Optimization of mesoporous titanosilicate catalysts for cyclohexene epoxidation via statistically guided synthesis.Journal of materials science · 2018Article
- A reductive aminase from Aspergillus oryzae.Nature chemistry · 2017Article
- Characterization of Carboxylic Acid Reductases as Enzymes in the Toolbox for Synthetic Chemistry.ChemCatChem · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The expected product yield of a biocatalyst during its useful lifetime is an important consideration when designing a continuous biocatalytic process. One important indicator of lifetime biocatalyst productivity is the dimensionless total turnover number (TTN). Here, a method is proposed for estimating the TTN of a given biocatalyst from readily-measured biochemical quantities, namely the specific activity and the deactivation half-life, measured under identical conditions. We demonstrate that this method may be applied to any enzyme whose thermal deactivation follows first-order kinetics, regardless of the number of unfolding intermediates, and that the TTN method circumvents the potential problems associated with measuring specific catalyst output when a portion of the enzyme is already unfolded. The TTN estimation was applied to several representative biocatalysts to demonstrate its applicability in identifying the most cost-effective catalyst from a pool of engineered mutants with similar activity and thermal stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.