ArticlePloS one2010
A stem cell-based tool for small molecule screening in adipogenesis.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- High-throughput, nonperturbing quantification of lipid droplets with digital holographic microscopy.Journal of lipid research · 2018Article
- Pyrintegrin Induces Soft Tissue Formation by Transplanted or Endogenous Cells.Scientific reports · 2017Article
- Therapeutic Potential of Differentiated Mesenchymal Stem Cells for Treatment of Osteoarthritis.International journal of molecular sciences · 2015Review
- MicroRNAs as novel regulators of stem cell fate.World journal of stem cells · 2013Review
- An engineered approach to stem cell culture: automating the decision process for real-time adaptive subculture of stem cells.PloS one · 2011Article
Corrections and comments
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Authors and funding
10 authors.
Funding
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Abstract
Techniques for small molecule screening are widely used in biological mechanism study and drug discovery. Here, we reported a novel adipocyte differentiation assay for small molecule selection, based on human mesenchymal stem cells (hMSCs) transduced with fluorescence reporter gene driven by adipogenic specific promoter--adipocyte Protein 2 (aP2; also namely Fatty Acid Binding Protein 4, FABP4). During normal adipogenic induction as well as adipogenic inhibition by Ly294002, we confirmed that the intensity of green fluorescence protein corresponded well to the expression level of aP2 gene. Furthermore, this variation of green fluorescence protein intensity can be read simply through fluorescence spectrophotometer. By testing another two small molecules in adipogenesis--Troglitazone and CHIR99021, we proved that this is a simple and sensitive method, which could be applied in adipocyte biology, drug discovery and toxicological study in the future.
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Registered trials
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