ArticleNature protocols2010
A high-throughput method for the quantitative analysis of auxins.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Analytical methods for stable isotope labeling to elucidate rapid auxin kinetics in Arabidopsis thaliana.PloS one · 2024Article
- Can plant hormonomics be built on simple analysis? A review.Plant methods · 2023Review
- Effect of ion source polarity and dopants on the detection of auxin plant hormones by ion mobility-mass spectrometry.Analytical and bioanalytical chemistry · 2022Article
- Biphasic control of cell expansion by auxin coordinates etiolated seedling development.Science advances · 2022Article
- Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis.Plant methods · 2021Article
- Metamaterial architecture from a self-shaping carnivorous plant.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Negative Regulation of Age-Related Developmental Leaf Senescence by the IAOx Pathway, PEN1, and PEN3.Frontiers in plant science · 2019Article
- Auxin analysis using laser microdissected plant tissues sections.BMC plant biology · 2018Article
- Single-Molecule Fluorescence Methods to Study Plant Hormone Signal Transduction Pathways.Frontiers in plant science · 2017Article
- A Simple, Rapid Method for Determination of Melatonin in Plant Tissues by UPLC Coupled with High Resolution Orbitrap Mass Spectrometry.Frontiers in plant science · 2017Article
- Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins.Biological procedures online · 2017Article
- A Quantitative Profiling Method of Phytohormones and Other Metabolites Applied to Barley Roots Subjected to Salinity Stress.Frontiers in plant science · 2016Article
- Auxin and Tryptophan Homeostasis Are Facilitated by the ISS1/VAS1 Aromatic Aminotransferase in Arabidopsis.Genetics · 2015Article
- Quo vadis plant hormone analysis?Planta · 2014Review
- Article
- A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics.Scientific reports · 2013Article
- Role for apyrases in polar auxin transport in Arabidopsis.Plant physiology · 2012Article
- Protocol: High-throughput and quantitative assays of auxin and auxin precursors from minute tissue samples.Plant methods · 2012Article
- Transport of indole-3-butyric acid and indole-3-acetic acid in Arabidopsis hypocotyls using stable isotope labeling.Plant physiology · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Auxin measurements in plants are critical to understanding both auxin signaling and metabolic homeostasis. The most abundant natural auxin is indole-3-acetic acid (IAA). This protocol is for the precise, high-throughput determination of free IAA in plant tissue by isotope dilution analysis using gas chromatography-mass spectrometry (GC-MS). The steps described are as follows: harvesting of plant material; amino and polymethylmethacrylate solid-phase purification followed by derivatization with diazomethane (either manual or robotic); GC-MS analysis; and data analysis. [¹³C₆]IAA is the standard used. The amount of tissue required is relatively small (25 mg of fresh weight) and one can process more than 500 samples per week using an automated system. To extract eight samples, this procedure takes ∼3 h, whether performed manually or robotically. For processing more than eight samples, robotic extraction becomes substantially more time efficient, saving at least 0.5 h per additional batch of eight samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.