ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2010
Glutamine protects against apoptosis via downregulation of Sp3 in intestinal epithelial cells.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Impact of Glutamine-Enhanced Parenteral Nutrition on Postoperative Outcomes in Colorectal Cancer Patients.Cancer management and research · 2024Article
- Association study reveals a susceptibility locus with male pattern baldness in the Han Chinese population.Frontiers in genetics · 2024Article
- Dietary Glutamine Inclusion Regulates Immune and Antioxidant System, as Well as Programmed Cell Death in Fish to Protect againstAntioxidants (Basel, Switzerland) · 2021Article
- SP and KLF Transcription Factors in Digestive Physiology and Diseases.Gastroenterology · 2017Review
- A CRISPR/Cas9 Functional Screen Identifies Rare Tumor Suppressors.Scientific reports · 2016Article
- Article
- Pathophysiological mechanisms of death resistance in colorectal carcinoma.World journal of gastroenterology · 2015Review
- Gene expression during zombie ant biting behavior reflects the complexity underlying fungal parasitic behavioral manipulation.BMC genomics · 2015Article
- Glutamine and alanyl-glutamine promote crypt expansion and mTOR signaling in murine enteroids.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Long-term intermittent glutamine supplementation repairs intestinal damage (structure and functional mass) with advanced age: assessment with plasma citrulline in a rodent model.The journal of nutrition, health & aging · 2014Article
- Oxygen in the regulation of intestinal epithelial transport.The Journal of physiology · 2014Review
- The canonical Notch signaling was involved in the regulation of intestinal epithelial cells apoptosis after intestinal ischemia/reperfusion injury.International journal of molecular sciences · 2014Article
- Syndecan 1 plays a novel role in enteral glutamine's gut-protective effects of the postischemic gut.Shock (Augusta, Ga.) · 2012Article
- Arginine decreases peroxisome proliferator-activated receptor-γ activity via c-Jun.Molecular and cellular biochemistry · 2012Article
- Alanyl-glutamine promotes intestinal epithelial cell homeostasis in vitro and in a murine model of weanling undernutrition.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Glutamine activates peroxisome proliferator-activated receptor-γ in intestinal epithelial cells via 15-S-HETE and 13-OXO-ODE: a novel mechanism.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Glutamine plays a key role in intestinal growth and maintenance of gut function, and as we have shown protects the postischemic gut (Kozar RA, Scultz SG, Bick RJ, Poindexter BJ, Desoigne R, Weisbrodt NW, Haber MM, Moore FA. Shock 21: 433-437, 2004). However, the precise mechanisms of the gut protective effects of glutamine have not been well elucidated. In the present study, RNA microarray was performed to obtain differentially expressed genes in intestinal epithelial IEC-6 cells following either 2 mM or 10 mM glutamine. The result demonstrated that specificity protein 3 (Sp3) mRNA expression was downregulated 3.1-fold. PCR and Western blot confirmed that Sp3 expression was decreased by glutamine in a time- and dose-dependent fashion. To investigate the role of Sp3, Sp3 gene siRNA silencing was performed and apoptosis was assessed. Silencing of Sp3 demonstrated a significant increase in Bcl-2 and decrease in Bax protein expression, as well as a decrease in caspase-3, -8, and -9 protein expression and activity. The protein expression of apoptosis-related proteins after hypoxia/reoxygenation was similar to that of normoxia and correlated with a decrease in DNA fragmentation. Importantly, the addition of glutamine to Sp3-silenced cells did not further lessen apoptosis, suggesting that Sp3 plays a major role in the inhibitory effect of glutamine on apoptosis. This novel finding may explain in part the gut-protective effects of glutamine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.