ArticleJournal of lipid research2011
In vivo D2O labeling to quantify static and dynamic changes in cholesterol and cholesterol esters by high resolution LC/MS.
Article in Journal of lipid research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- Turnover Rate of Lipids, Metabolites and Proteins Revealed by 156-Day-Long DInternational journal of molecular sciences · 2026Article
- Aerobic Capacity and Exercise Mediate Protection Against Hepatic Steatosis via Enhanced Bile Acid Metabolism.Function (Oxford, England) · 2025Article
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Bottlenecks in the Investigation of Retinal Sterol Homeostasis.Biomolecules · 2024Review
- Investigating the Metabolism of Plants Germinated in Heavy Water, DInternational journal of molecular sciences · 2023Article
- Metabolomics and lipidomics strategies in modern drug discovery and development.Drug discovery today · 2023Review
- Measurement of lipid flux to advance translational research: evolution of classic methods to the future of precision health.Experimental & molecular medicine · 2022Review
- A Boost in Mitochondrial Activity Underpins the Cholesterol-Lowering Effect of Annurca Apple Polyphenols on Hepatic Cells.Nutrients · 2019Article
- A chemical biology screen identifies a vulnerability of neuroendocrine cancer cells to SQLE inhibition.Nature communications · 2019Article
- Analytical Considerations of Stable Isotope Labelling in Lipidomics.Biomolecules · 2018Review
- Stable isotope tracers and exercise physiology: past, present and future.The Journal of physiology · 2017Review
- Increased lanosterol turnover: a metabolic burden for daunorubicin-resistant leukemia cells.Medical oncology (Northwood, London, England) · 2016Article
- Rewired metabolism in drug-resistant leukemia cells: a metabolic switch hallmarked by reduced dependence on exogenous glutamine.The Journal of biological chemistry · 2015Article
- Stable isotope-labeling studies in metabolomics: new insights into structure and dynamics of metabolic networks.Bioanalysis · 2014Review
- Measurement of apo(a) kinetics in human subjects using a microfluidic device with tandem mass spectrometry.Rapid communications in mass spectrometry : RCM · 2013Article
- Tracking fatty acid kinetics in distinct lipoprotein fractions in vivo: a novel high-throughput approach for studying dyslipidemia in rodent models.Journal of lipid research · 2013Article
- Quantifying apoprotein synthesis in rodents: coupling LC-MS/MS analyses with the administration of labeled water.Journal of lipid research · 2012Article
- Dietary regulation of catabolic disposal of 4-hydroxynonenal analogs in rat liver.Free radical biology & medicine · 2012Article
- Anacetrapib promotes reverse cholesterol transport and bulk cholesterol excretion in Syrian golden hamsters.Journal of lipid research · 2011Article
- ApoB siRNA-induced liver steatosis is resistant to clearance by the loss of fatty acid transport protein 5 (Fatp5).Lipids · 2011Article
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High resolution LC/MS-MS and LC/APPI-MS methods have been established for the quantitation of flux in the turnover of cholesterol and cholesterol ester. Attention was directed toward quantifying the monoisotopic mass (M0) and that of the singly deuterated labeled (M+1) isotope. A good degree of isotopic dynamic range has been achieved by LC/MS-MS ranging from 3-4 orders of magnitude. Correlation between the linearity of GC/MS and LC atmospheric pressure photoionization (APPI)-MS are complimentary (r² = 0.9409). To prove the viability of this particular approach, male C57Bl/6 mice on either a high carbohydrate (HC) or a high fat (HF) diet were treated with ²H₂O for 96 h. Gene expression analysis showed an increase in the activity of stearoyl-CoA desaturase (Scd1) in the HC diet up to 69-fold (P < 0.0008) compared with the HF diet. This result was supported by the quantitative flux measurement of the isotopic incorporation of ²H into the respective cholesterol and cholesterol ester (CE) pools. We concluded that it is possible to readily obtain static and dynamic measurement of cholesterol and CEs in vivo by coupling novel LC/MS methods with stable isotope-based protocols.
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