ArticleThe Plant cell2010
A novel motif essential for SNARE interaction with the K(+) channel KC1 and channel gating in Arabidopsis.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed.
- A MAP-SNARE interaction links cortical microtubules to the exocytic fusion machinery during auxin-dependent growth remodeling.Science advances · 2026Article
- COJournal of integrative plant biology · 2026Article
- A guard cell carbonic anhydrase binds and regulates SLAC1 separate from its catalytic activity.Nature communications · 2026Article
- GORK KNature communications · 2025Article
- The clues offered by SNAREs on the vacuoles of plants and animals.Frontiers in plant science · 2025Review
- SYNTAXIN OF PLANTS 132 underpins secretion of cargoes associated with salicylic acid signaling and pathogen defense.Plant physiology · 2024Article
- Bestrophin-like protein 4 is involved in photosynthetic acclimation to light fluctuations in Chlamydomonas.Plant physiology · 2024Article
- Research Progress on Plant Shaker KPlants (Basel, Switzerland) · 2024Review
- A charged existence: A century of transmembrane ion transport in plants.Plant physiology · 2024Review
- Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants.Antioxidants (Basel, Switzerland) · 2024Review
- 2 in 1 Vectors Improve in Planta BiFC and FRET Analysis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- VAMP726 and VAMP725 regulate vesicle secretion and pollen tube growth in Arabidopsis.Plant cell reports · 2023Article
- Analyzing Protein-Protein Interactions Using the Split-Ubiquitin System.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Phosphorylation of S11 in PHR1 negatively controls its transcriptional activity.Physiologia plantarum · 2022Article
- Engineering a KNature plants · 2022Article
- A mitochondrial ADXR-ADX-P450 electron transport chain is essential for maternal gametophytic control of embryogenesis inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Identification and Characterization of Shaker KFrontiers in plant science · 2022Article
- The sorting of cargo proteins in the plantFrontiers in plant science · 2022Review
- Molecular Evolution of Calcium Signaling and Transport in Plant Adaptation to Abiotic Stress.International journal of molecular sciences · 2021Review
- Genome-wide dynamic network analysis reveals the potential genes for MeJA-induced growth-to-defense transition.BMC plant biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The SNARE (for soluble N-ethylmaleimide-sensitive factor protein attachment protein receptor) protein SYP121 (=SYR1/PEN1) of Arabidopsis thaliana facilitates vesicle traffic, delivering ion channels and other cargo to the plasma membrane, and contributing to plant cell expansion and defense. Recently, we reported that SYP121 also interacts directly with the K(+) channel subunit KC1 and forms a tripartite complex with a second K(+) channel subunit, AKT1, to control channel gating and K(+) transport. Here, we report isolating a minimal sequence motif of SYP121 prerequisite for its interaction with KC1. We made use of yeast mating-based split-ubiquitin and in vivo bimolecular fluorescence complementation assays for protein-protein interaction and of expression and electrophysiological analysis. The results show that interaction of SYP121 with KC1 is associated with a novel FxRF motif uniquely situated within the first 12 residues of the SNARE sequence, that this motif is the minimal requirement for SNARE-dependent alterations in K(+) channel gating when heterologously expressed, and that rescue of KC1-associated K(+) current of the root epidermis in syp121 mutant Arabidopsis plants depends on expression of SNARE constructs incorporating this motif. These results establish the FxRF sequence as a previously unidentified motif required for SNARE-ion channel interactions and lead us to suggest a mechanistic framework for understanding the coordination of vesicle traffic with transmembrane ion transport.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.