ArticleThe Journal of biological chemistry2010
Cellular abundance of Mps1 and the role of its carboxyl terminal tail in substrate recruitment.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Identifying the critical states and dynamic network biomarkers of cancers based on network entropy.Journal of translational medicine · 2022Article
- The essential roles of Mps1 in spermatogenesis and fertility in mice.Cell death & disease · 2021Article
- Mps1 dimerization and multisite interactions with Ndc80 complex enable responsive spindle assembly checkpoint signaling.Journal of molecular cell biology · 2020Article
- TC Mps1 12, a novel Mps1 inhibitor, suppresses the growth of hepatocellular carcinoma cells via the accumulation of chromosomal instability.British journal of pharmacology · 2017Article
- Genetic and pharmacological inhibition of TTK impairs pancreatic cancer cell line growth by inducing lethal chromosomal instability.PloS one · 2017Article
- Positive Feedback Keeps Duration of Mitosis Temporally Insulated from Upstream Cell-Cycle Events.Molecular cell · 2016Article
- Association between polymorphisms in segregation genes BUB1B and TTK and gastric cancer risk.Radiology and oncology · 2016Article
- Modulation of miR-21 signaling by MPS1 in human glioblastoma.Oncotarget · 2016Article
- Mps1 kinase regulates tumor cell viability via its novel role in mitochondria.Cell death & disease · 2016Article
- Mps1 is SUMO-modified during the cell cycle.Oncotarget · 2016Article
- Targeting MPS1 Enhances Radiosensitization of Human Glioblastoma by Modulating DNA Repair Proteins.Molecular cancer research : MCR · 2015Article
- A motif from Lys216 to Lys222 in human BUB3 protein is a nuclear localization signal and critical for BUB3 function in mitotic checkpoint.The Journal of biological chemistry · 2015Article
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- Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1.Molecular biology of the cell · 2012Article
- The MPS1 family of protein kinases.Annual review of biochemistry · 2012Review
- Two LXXLL motifs in the N terminus of Mps1 are required for Mps1 nuclear import during G(2)/M transition and sustained spindle checkpoint responses.Cell cycle (Georgetown, Tex.) · 2011Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Mps1 is a protein kinase that regulates normal mitotic progression and the spindle checkpoint in response to spindle damage. The levels of Mps1 are relatively low in cells during interphase but elevated in mitosis or upon activation of the spindle checkpoint, although the dynamic range of Mps1 expression and the Mps1 catalytic mechanism have not been carefully characterized. Our recent structural studies of the Mps1 kinase domain revealed that the carboxyl-terminal tail region of Mps1 is unstructured, raising the question of whether this region has any functional role in Mps1 catalysis. Here we first determined the cellular abundance of Mps1 during cell cycle progression and found that Mps1 levels vary between 60,000 per cell in early G(1) and 110,000 per cell during mitosis. We studied phosphorylation of a number of Mps1 substrates in vitro and in culture cells. Unexpectedly, we found that the unstructured carboxyl-terminal region of Mps1 plays an essential role in substrate recruitment. Kinetics studies using the purified recombinant wild type and mutant kinases indicate that the carboxyl-terminal tail is largely dispensable for autophosphorylation of Mps1 but critical for trans-phosphorylation of substrates in vitro and in cultured cells. Mps1 mutant without the unstructured tail region is defective in mediating spindle assembly checkpoint activation. Our results underscore the importance of the unstructured tail region of Mps1 in kinase activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.