ArticleThe Journal of biological chemistry2010
31P saturation transfer spectroscopy predicts differential intracellular macromolecular association of ATP and ADP in skeletal muscle.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 33 citations in OpenAlex.
- 3DNMR in biomedicine · 2021Article
- Quantification of creatine kinase reaction rate in mouse hindlimb using phosphorus-31 magnetic resonance spectroscopic fingerprinting.NMR in biomedicine · 2021Article
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- ModularMagnetic resonance in medicine · 2019Article
- A Mouse Model of Creatine Transporter Deficiency Reveals Impaired Motor Function and Muscle Energy Metabolism.Frontiers in physiology · 2018Article
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- EfficientMagnetic resonance in medicine · 2017Article
- Band inversion amplifiesMagnetic resonance in medicine · 2017Article
- A simple approach to evaluate the kinetic rate constant for ATP synthesis in resting human skeletal muscle at 7 T.NMR in biomedicine · 2016Article
- Amplification of the effects of magnetization exchange by (31) P band inversion for measuring adenosine triphosphate synthesis rates in human skeletal muscle.Magnetic resonance in medicine · 2015Article
- Exchange kinetics by inversion transfer: integrated analysis of the phosphorus metabolite kinetic exchanges in resting human skeletal muscle at 7 T.Magnetic resonance in medicine · 2015Article
- Modular organization of cardiac energy metabolism: energy conversion, transfer and feedback regulation.Acta physiologica (Oxford, England) · 2015Review
- Human cardiac 31P magnetic resonance spectroscopy at 7 Tesla.Magnetic resonance in medicine · 2014Article
- Gene transfer of arginine kinase to skeletal muscle using adeno-associated virus.Gene therapy · 2014Article
- What do magnetic resonance-based measurements of Pi→ATP flux tell us about skeletal muscle metabolism?Diabetes · 2012Review
- Dynamic phosphometabolomic profiling of human tissues and transgenic models by 18O-assisted ³¹P NMR and mass spectrometry.Physiological genomics · 2012Article
- Phosphocreatine interacts with phospholipids, affects membrane properties and exerts membrane-protective effects.PloS one · 2012Article
- Molecular system bioenergics of the heart: experimental studies of metabolic compartmentation and energy fluxes versus computer modeling.International journal of molecular sciences · 2011Review
- Exploration of New Contrasts, Targets, and MR Imaging and Spectroscopy Techniques for Neuromuscular Disease - A Workshop Report of Working Group 3 of the Biomedicine and Molecular Biosciences COST Action BM1304 MYO-MRI.Journal of neuromuscular diseasesArticle
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The kinetics of phosphoryl exchange involving ATP and ADP have been investigated successfully by in vivo (31)P magnetic resonance spectroscopy using magnetization transfer. However, magnetization transfer effects seen on the signals of ATP also could arise from intramolecular cross-relaxation. This relaxation process carries information on the association state of ATP in the cell. To disentangle contributions of chemical exchange and cross-relaxation to magnetization transfer effects seen in (31)P magnetic resonance spectroscopy of skeletal muscle, we performed saturation transfer experiments on wild type and double-mutant mice lacking the cytosolic muscle creatine kinase and adenylate kinase isoforms. We find that cross-relaxation, observed as nuclear Overhauser effects (NOEs), is responsible for magnetization transfer between ATP phosphates both in wild type and in mutant mice. Analysis of (31)P relaxation properties identifies these effects as transferred NOEs, i.e. underlying this process is an exchange between free cellular ATP and ATP bound to slowly rotating macromolecules. This explains the β-ATP signal decrease upon saturation of the γ-ATP resonance. Although this usually is attributed to β-ADP ↔ β-ATP phosphoryl exchange, we did not detect an effect of this exchange on the β-ATP signal as expected for free [ADP], derived from the creatine kinase equilibrium reaction. This indicates that in resting muscle, conditions prevail that prevent saturation of β-ADP spins and puts into question the derivation of free [ADP] from the creatine kinase equilibrium. We present a model, matching the experimental result, for ADP ↔ ATP exchange, in which ADP is only transiently present in the cytosol.
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