ArticleBiotechnology letters2011
Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection.
Article in Biotechnology letters, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Development of an automated chemiluminescence immunoassay for detection of IgA antibodies against porcine epidemic diarrhea virus spike.BMC veterinary research · 2026Article
- The structure and function of membrane protein in coronavirus infection and its applications in the development of vaccines and therapeutic drugs.Frontiers in microbiology · 2026Review
- Surface displayed porcine epidemic diarrhea virus membrane epitopes onJournal of animal science and technology · 2025Article
- Induction of Immune Responses in Mice and Newborn Piglets by Oral Immunization with RecombinantMicroorganisms · 2025Article
- Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.Discover nano · 2025Review
- Review
- Article
- Current State of Molecular and Serological Methods for Detection of Porcine Epidemic Diarrhea Virus.Pathogens (Basel, Switzerland) · 2022Review
- Development of an indirect ELISA to detect PEDV specific IgA antibody based on a PEDV epidemic strain.BMC veterinary research · 2022Article
- Porcine Enteric Coronavirus Infections in Wild Boar in Poland - a Pilot Study.Journal of veterinary research · 2021Article
- PEDV Infection Generates Conformation-Specific Antibodies That Can Be Effectively Detected by a Cell-Based ELISA.Viruses · 2021Article
- Development and comparison of enzyme-linked immunosorbent assays based on recombinant trimeric full-length and truncated spike proteins for detecting antibodies against porcine epidemic diarrhea virus.BMC veterinary research · 2019Article
- Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus.Applied microbiology and biotechnology · 2018Article
- Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea Virus.BMC veterinary research · 2015Article
- Porcine epidemic diarrhea: a review of current epidemiology and available vaccines.Clinical and experimental vaccine research · 2015Review
- Analysis of protein expression changes of the Vero E6 cells infected with classic PEDV strain CV777 by using quantitative proteomic technique.Journal of virological methods · 2015Article
- Detection of immunoglobulin (Ig) A antibodies against porcine epidemic diarrhea virus (PEDV) in fecal and serum samples.MethodsX · 2015Article
- A phage-displayed peptide recognizing porcine aminopeptidase N is a potent small molecule inhibitor of PEDV entry.Virology · 2014Article
- Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses.Virology journal · 2013Article
- Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines.Virus genes · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A membrane (M), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The M gene of PEDV was expressed in Escherichia coli. The purified recombinant M protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the anti-PEDV-M antibody reacted with PEDV-infected cells. The antibody was utilized to develop an indirect ELISA to detect PEDV. Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.