ArticleLeukemia2010
Diagnosis of invasive fungal infections by a real-time panfungal PCR assay in immunocompromised pediatric patients.
Article in Leukemia, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02492594 (Optimized Diagnostics for Improved Therapy Stratification in Invasive Fungal Infections), which is not on this map. Cited by 22 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Optimized Diagnostics for Improved Therapy Stratification in Invasive Fungal Infections
Who cites it
22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 84 citations in OpenAlex.
- Sensitivity and Specificity of Polymerase Chain Reaction in Blood and Bronchoalveolar Lavage Samples for Mucormycosis: A Bayesian Diagnostic Test Accuracy Meta-Analysis.Mycopathologia · 2025Pooled it
- Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people.The Cochrane database of systematic reviews · 2019Pooled it
- Molecular quantification and differentiation of Candida species in biological specimens of patients with liver cirrhosis.PloS one · 2018Trial
- The future of fungal diagnostics: What's next in laboratory testing for invasive fungal infection?Medical mycology · 2026Review
- Diagnostics for human pathogenic fungal infections: Current status and future prospects.hLife · 2026Review
- Diagnosis and management of invasive fungal infections due to non-Aspergillus moulds.The Journal of antimicrobial chemotherapy · 2025Review
- Prevalence of fungal DNAemia mediated by putatively non-pathogenic fungi in immunocompromised patients with febrile neutropenia: a prospective cohort study.Journal of hematology & oncology · 2024Article
- Main genome characteristics of butanol-producing Clostridium sp. UCM В-7570 strain.Journal of applied genetics · 2023Article
- Review
- Utility of Panfungal PCR in the diagnosis of invasive fungal infections in febrile neutropenia.Journal of family medicine and primary care · 2021Article
- Clinical evaluation of an in-house panfungal real-time PCR assay for the detection of fungal pathogens.Infection · 2020Article
- Detection and identification of fungi in bronchoalveolar lavage fluid from immunocompromised patients using panfungal PCR.Folia microbiologica · 2019Article
- A New Age in Molecular Diagnostics for Invasive Fungal Disease: Are We Ready?Frontiers in microbiology · 2019Review
- Prospective Biomarker Screening for Diagnosis of Invasive Aspergillosis in High-Risk Pediatric Patients.Journal of clinical microbiology · 2017Article
- Progress in the Diagnosis of Invasive Fungal Disease in Children.Current fungal infection reports · 2017Review
- New Panfungal Real-Time PCR Assay for Diagnosis of Invasive Fungal Infections.Journal of clinical microbiology · 2016Article
- Discrimination of Aspergillosis, Mucormycosis, Fusariosis, and Scedosporiosis in Formalin-Fixed Paraffin-Embedded Tissue Specimens by Use of Multiple Real-Time Quantitative PCR Assays.Journal of clinical microbiology · 2016Article
- Similar efficacy of broad-range ITS PCR and conventional fungal culture for diagnosing fungal infections in non-immunocompromised patients.BMC microbiology · 2016Article
- Occurrence of Fungal DNA Contamination in PCR Reagents: Approaches to Control and Decontamination.Journal of clinical microbiology · 2016Article
- Diagnostic Modalities for Invasive Mould Infections among Hematopoietic Stem Cell Transplant and Solid Organ Recipients: Performance Characteristics and Practical Roles in the Clinic.Journal of fungi (Basel, Switzerland) · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invasive fungal disease (IFD) is a life-threatening event in immunocompromised patients, and there is an urgent need for reliable screening methods facilitating rapid and broad detection of pathogenic fungi. We have established a two-reaction real-time PCR assay permitting highly sensitive detection of more than 80 fungal pathogens, covering a large spectrum of moulds, yeasts and Zygomycetes. To assess the clinical potential of the assay, more than 600 consecutive specimens from 125 pediatric patients carrying a high risk of IFD were analyzed. An excellent correlation between PCR positivity and the presence of proven, probable or possible fungal infection according to the European Organization for Research and Treatment of Cancer criteria was demonstrated, as revealed by the sensitivity of the assay of 96% (95% CI: 82-99%). The negative predictive value of the panfungal PCR assay presented was 98% (95% CI: 90-100%), while the specificity and the positive predictive value were 77% (95% CI: 66-85%) and 62% (95% CI: 47-75%), respectively. The results indicate that molecular screening of patients during febrile neutropenic episodes by the assay presented could help prevent unnecessary toxicity resulting from empirical antifungal treatment in individuals who may not be at risk of imminent fungal disease. Our observations raise the possibility that rapid species identification may be required to increase the positive predictive value for impending fungus-related disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.