ArticleNucleic acids research2010
Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Mouse Models of HIV-Associated Atherosclerosis.International journal of molecular sciences · 2025Review
- Targeted insertion of conditional expression cassettes into the mouse genome using the modified i-PITT.BMC genomics · 2024Article
- Targeted Integration of Transgenes at the Mouse Gt(ROSA)26Sor Locus.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Dll1 Can Function as a Ligand of Notch1 and Notch2 in the Thymic Epithelium.Frontiers in immunology · 2022Article
- Murine SEC24D can substitute functionally for SEC24C during embryonic development.Scientific reports · 2021Article
- Novel reporter mouse models useful for evaluatingMolecular therapy. Nucleic acids · 2021Article
- Designing and generating a mouse model: frequently asked questions.Journal of biomedical research · 2021Article
- Thy1 promoter activity in the Rosa26 locus in mice: lessons from Dre-rox conditional expression system.Experimental animals · 2020Article
- Article
- Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD.Nature protocols · 2019Article
- In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression.Journal of visualized experiments : JoVE · 2019Article
- Generating mouse models for biomedical research: technological advances.Disease models & mechanisms · 2019Review
- Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches.Scientific reports · 2018Article
- Cattle with a precise, zygote-mediated deletion safely eliminate the major milk allergen beta-lactoglobulin.Scientific reports · 2018Article
- Cell-cell interactions between monocytes/macrophages and synoviocyte-like cells promote inflammatory cell infiltration mediated by augmentation of MCP-1 production in temporomandibular joint.Bioscience reports · 2018Article
- Pronuclear Injection-Based Targeted Transgenesis.Current protocols in human genetics · 2016Article
- Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new.Biology direct · 2016Review
- Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes.BMC biotechnology · 2016Article
- Article
- A versatile transgenic allele for mouse overexpression studies.Mammalian genome : official journal of the International Mammalian Genome Society · 2015Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mouse transgenesis has proven invaluable for analysis of gene function and generation of human disease models. We describe here the development of a pronuclear injection-based targeted transgenesis (PITT) system, involving site-specific integration in fertilized eggs. The system was applied to two different genomic target loci to generate a series of transgenic lines including fluorescent mice, which reproducibly displayed strong, ubiquitous and stable transgene expression. We also demonstrated that knockdown mice could be readily generated by PITT by taking advantage of the reproducible and highly efficient expression system. The PITT system, which circumvents the problem of unpredictable and unstable transgene expression of conventional random-integration transgenic mice, reduces the time, cost and effort needed to generate transgenic mice, and is potentially applicable to both in vivo 'gain-of-function' and 'loss-of-function' studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.