ArticleThe Journal of biological chemistry2010
Time-resolved measurements of intracellular ATP in the yeast Saccharomyces cerevisiae using a new type of nanobiosensor.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed, 118 citations in OpenAlex.
- Inositol biosynthesis is inversely regulated by glycolytic activity.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2026Article
- Proteasome-dependent degradation and nucleus-vacuole junctions sustain proteostasis during acute glucose starvation.bioRxiv : the preprint server for biology · 2026Article
- An optical BOD biosensor based on intracellular ATP measurements in genetically modified Saccharomyces cerevisiae.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- The role of hexokinases in epigenetic regulation: altered hexokinase expression and chromatin stability in yeast.Epigenetics & chromatin · 2024Article
- From biological data to oscillator models using SINDy.iScience · 2024Article
- D-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Confocal imaging of biomarkers at a single-cell resolution: quantifying 'living' in 3D-printable engineered living material based on Pluronic F-127 and yeast Saccharomyces cerevisiae.Biomaterials research · 2022Article
- Intramitochondrial co-assembly between ATP and nucleopeptides induces cancer cell apoptosis.Chemical science · 2022Article
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- Modeling and analysis of the macronutrient signaling network in budding yeast.Molecular biology of the cell · 2021Article
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- Evolution of the human mitochondrial ABCB7 [2Fe-2S](GS)Archives of biochemistry and biophysics · 2021Article
- Self-Organization and Information Processing: From Basic Enzymatic Activities to Complex Adaptive Cellular Behavior.Frontiers in genetics · 2021Review
- Partial synchronisation of glycolytic oscillations in yeast cell populations.Scientific reports · 2020Article
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Engineering base-excised aptamers for highly specific recognition of adenosine.Chemical science · 2020Article
- Modeling Cell Energy Metabolism as Weighted Networks of Non-autonomous Oscillators.Frontiers in physiology · 2020Article
- Coupled Response of Membrane Hydration with Oscillating Metabolism in Live Cells: An Alternative Way to Modulate Structural Aspects of Biological Membranes?Biomolecules · 2019Article
- An A666G mutation in transmembrane helix 5 of the yeast multidrug transporter Pdr5 increases drug efflux by enhancing cooperativity between transport sites.Molecular microbiology · 2019Article
- Import of extracellular ATP in yeast and man modulates AMPK and TORC1 signalling.Journal of cell science · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adenosine 5'-triphosphate is a universal molecule in all living cells, where it functions in bioenergetics and cell signaling. To understand how the concentration of ATP is regulated by cell metabolism and in turn how it regulates the activities of enzymes in the cell it would be beneficial if we could measure ATP concentration in the intact cell in real time. Using a novel aptamer-based ATP nanosensor, which can readily monitor intracellular ATP in eukaryotic cells with a time resolution of seconds, we have performed the first on-line measurements of the intracellular concentration of ATP in the yeast Saccharomyces cerevisiae. These ATP measurements show that the ATP concentration in the yeast cell is not stationary. In addition to an oscillating ATP concentration, we also observe that the concentration is high in the starved cells and starts to decrease when glycolysis is induced. The decrease in ATP concentration is shown to be caused by the activity of membrane-bound ATPases such as the mitochondrial F(0)F(1) ATPase-hydrolyzing ATP and the plasma membrane ATPase (PMA1). The activity of these two ATPases are under strict control by the glucose concentration in the cell. Finally, the measurements of intracellular ATP suggest that 2-deoxyglucose (2-DG) may have more complex function than just a catabolic block. Surprisingly, addition of 2-DG induces only a moderate decline in ATP. Furthermore, our results suggest that 2-DG may inhibit the activation of PMA1 after addition of glucose.
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