ArticleMolecular microbiology2010
The FACT subunit TbSpt16 is involved in cell cycle specific control of VSG expression sites in Trypanosoma brucei.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
- Unwrap RAP1's Mystery at Kinetoplastid Telomeres.Biomolecules · 2024Review
- Shared Mechanisms for Mutually Exclusive Expression and Antigenic Variation by Protozoan Parasites.Frontiers in cell and developmental biology · 2022Review
- Regulation of Antigenic Variation byPathogens (Basel, Switzerland) · 2021Review
- Telomere and Subtelomere R-loops and Antigenic Variation in Trypanosomes.Journal of molecular biology · 2020Review
- Trypanosoma brucei ATR Links DNA Damage Signaling during Antigenic Variation with Regulation of RNA Polymerase I-Transcribed Surface Antigens.Cell reports · 2020Article
- SUMOylated SNF2PH promotes variant surface glycoprotein expression in bloodstream trypanosomes.EMBO reports · 2019Article
- Epigenetic Regulation of Transcription in Trypanosomatid Protozoa.Current genomics · 2018Review
- Transcriptional Regulation of Telomeric Expression Sites and Antigenic Variation in Trypanosomes.Current genomics · 2018Review
- How to create coats for all seasons: elucidating antigenic variation in African trypanosomes.Emerging topics in life sciences · 2017Article
- Well-positioned nucleosomes punctuate polycistronic pol II transcription units and flank silentEpigenetics & chromatin · 2017Article
- Epigenetic Regulation of Virulence Gene Expression in Parasitic Protozoa.Cell host & microbe · 2016Review
- Inositol phosphate pathway controls transcription of telomeric expression sites in trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- DNA double-strand breaks and telomeres play important roles in trypanosoma brucei antigenic variation.Eukaryotic cell · 2015Review
- Review
- SUMOylation by the E3 ligase TbSIZ1/PIAS1 positively regulates VSG expression in Trypanosoma brucei.PLoS pathogens · 2014Article
- FACT plays a major role in histone dynamics affecting VSG expression site control in Trypanosoma brucei.Molecular microbiology · 2014Article
- Trypanosoma brucei histone H1 inhibits RNA polymerase I transcription and is important for parasite fitness in vivo.Molecular microbiology · 2014Article
- Transcription is initiated on silent variant surface glycoprotein expression sites despite monoallelic expression in Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Promoter occupancy of the basal class I transcription factor A differs strongly between active and silent VSG expression sites in Trypanosoma brucei.Nucleic acids research · 2014Article
- Antigenic variation in African trypanosomes: the importance of chromosomal and nuclear context in VSG expression control.Cellular microbiology · 2013Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The African trypanosome Trypanosoma brucei monoallelically expresses one of more than 1000 Variant Surface Glycoprotein (VSG) genes. The active VSG is transcribed from one of about 15 telomeric VSG expression sites (ESs). It is unclear how monoallelic expression of VSG is controlled, and how inactive VSG ESs are silenced. Here, we show that blocking synthesis of the T. brucei FACT subunit TbSpt16 triggers a G2/early M phase cell cycle arrest in both bloodstream and insect form T. brucei. Segregation of T. brucei minichromosomes in these stalled cells is impaired, implicating FACT in maintenance of centromeres. Strikingly, knock-down of TbSpt16 results in 20- to 23-fold derepression of silent VSG ES promoters in bloodstream form T. brucei, with derepression specific to the G2/M cell cycle stage. In insect form T. brucei TbSpt16 knock-down results in 16- to 25-fold VSG ES derepression. Using chromatin immunoprecipitation (ChIP), TbSpt16 was found to be particularly enriched at the promoter region of silent but not active VSG ESs in bloodstream form T. brucei. The chromatin remodeler FACT is therefore implicated in maintenance of repressed chromatin present at silent VSG ES promoters, but is also essential for chromosome segregation presumably through maintenance of functional centromeres.
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