ArticleAnalytical chemistry2010
Quantitative analysis of blood plasma metabolites using isotope enhanced NMR methods.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- Influence of Glycemic Control on Endogenous Circulating Ketone Concentrations in Adults Following Traumatic Brain Injury.Neurocritical care · 2017Trial
- Multiplatform untargeted metabolomics.Magnetic resonance in chemistry : MRC · 2023Review
- Practical Aspects of NMR-Based Metabolomics.Handbook of experimental pharmacology · 2023Article
- Rapid quantitativeRSC advances · 2022Article
- Lactate supplementation in severe traumatic brain injured adults by primed constant infusion of sodium L-lactate.Journal of neuroscience research · 2018Article
- Beyond the paradigm: Combining mass spectrometry and nuclear magnetic resonance for metabolomics.Progress in nuclear magnetic resonance spectroscopy · 2017Review
- Comprehensive Metabolite Identification Strategy Using Multiple Two-Dimensional NMR Spectra of a Complex Mixture Implemented in the COLMARm Web Server.Analytical chemistry · 2016Article
- Analysis of human plasma metabolites across different liquid chromatography/mass spectrometry platforms: Cross-platform transferable chemical signatures.Rapid communications in mass spectrometry : RCM · 2016Article
- Applications of NMR spectroscopy to systems biochemistry.Progress in nuclear magnetic resonance spectroscopy · 2016Review
- Can NMR solve some significant challenges in metabolomics?Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Review
- Expanding the limits of human blood metabolite quantitation using NMR spectroscopy.Analytical chemistry · 2015Article
- Quantitating metabolites in protein precipitated serum using NMR spectroscopy.Analytical chemistry · 2014Article
- Multidimensional approaches to NMR-based metabolomics.Analytical chemistry · 2014Review
- Development of a Standard Reference Material for metabolomics research.Analytical chemistry · 2013Article
- 15N-cholamine--a smart isotope tag for combining NMR- and MS-based metabolite profiling.Analytical chemistry · 2013Article
- Combining Hydrophilic Interaction Chromatography (HILIC) and Isotope Tagging for Off-Line LC-NMR Applications in Metabolite Analysis.Metabolites · 2013Article
- Quantitative analysis of metabolic mixtures by two-dimensional 13C constant-time TOCSY NMR spectroscopy.Analytical chemistry · 2013Article
- Isotope enhanced approaches in metabolomics.Advances in experimental medicine and biology · 2012Review
- Ratio analysis nuclear magnetic resonance spectroscopy for selective metabolite identification in complex samples.Analytical chemistry · 2011Article
- Deconvolution of chemical mixtures with high complexity by NMR consensus trace clustering.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
NMR spectroscopy is a powerful analytical tool for both qualitative and quantitative analysis. However, accurate quantitative analysis in complex fluids such as human blood plasma is challenging, and analysis using one-dimensional NMR is limited by signal overlap. It is impractical to use heteronuclear experiments involving natural abundance (13)C on a routine basis due to low sensitivity, despite their improved resolution. Focusing on circumventing such bottlenecks, this study demonstrates the utility of a combination of isotope enhanced NMR experiments to analyze metabolites in human blood plasma. (1)H-(15)N HSQC and (1)H-(13)C HSQC experiments on the isotope tagged samples combined with the conventional (1)H one-dimensional and (1)H-(1)H TOCSY experiments provide quantitative information on a large number of metabolites in plasma. The methods were first tested on a mixture of 28 synthetic analogues of metabolites commonly present in human blood; 27 metabolites in a standard NIST (National Institute of Standards and Technology) human blood plasma were then identified and quantified with an average coefficient of variation of 2.4% for 17 metabolites and 5.6% when all the metabolites were considered. Carboxylic acids and amines represent a majority of the metabolites in body fluids, and their analysis by isotope tagging enables a significant enhancement of the metabolic pool for biomarker discovery applications. Improved sensitivity and resolution of NMR experiments imparted by (15)N and (13)C isotope tagging are attractive for both the enhancement of the detectable metabolic pool and accurate analysis of plasma metabolites. The approach can be easily extended to many additional metabolites in almost any biological mixture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.