ArticlePloS one2010
A blue spectral shift of the hemoglobin soret band correlates with the age (time since deposition) of dried bloodstains.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 107 citations in OpenAlex.
- Estimating time of day from fingertip blood samples using RNA molecules with diurnal oscillating expression: a proof-of-principle study.International journal of legal medicine · 2026Article
- Reconstruction Accuracy vs. Discriminative Power: Spectral Unmixing Performance in Brain Tissue Hyperspectral Imaging.Bioengineering (Basel, Switzerland) · 2026Article
- One trigger, two fates: hemolysate shapes neutrophil extracellular traps (NETs) formation in a context-dependent manner.Frontiers in immunology · 2026Article
- Influence of anticoagulant on the spectroscopic analysis of drying bovine blood pools.Journal of forensic sciences · 2025Article
- Raman Spectroscopy for the Time since Deposition Estimation of a Menstrual Bloodstain.Sensors (Basel, Switzerland) · 2024Article
- Assessment of Blood and Semen Detection and DNA Collection from Swabs up to Three Months after Deposition on Five Different Cloth Materials.International journal of molecular sciences · 2024Article
- Development of novel polymer haemoglobin based particles as an antioxidant, antibacterial and an oxygen carrier agents.Scientific reports · 2024Article
- Review
- Hyperspectral Imaging for Bloodstain Identification.Sensors (Basel, Switzerland) · 2021Article
- Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery.Biomedical optics express · 2021Article
- The fibrinolytic system enables the onset ofScience advances · 2021Article
- Insight into the Biological Activity of Hennosides-Glucosides Isolated fromPlants (Basel, Switzerland) · 2021Article
- Article
- Impacts of low concentration surfactant on red blood cell dielectrophoretic responses.Biomicrofluidics · 2019Article
- Article
- Design of a heme-binding peptide motif adopting a β-hairpin conformation.The Journal of biological chemistry · 2018Article
- Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon.Nano convergence · 2017Article
- Dating bloodstains with fluorescence lifetime measurements.Chemistry (Weinheim an der Bergstrasse, Germany) · 2012Article
- Injectable fibroblast growth factor-2 coacervate for persistent angiogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Biphasic oxidation of oxy-hemoglobin in bloodstains.PloS one · 2011Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to determine the time since deposition of a bloodstain found at a crime scene could prove invaluable to law enforcement investigators, defining the time frame in which the individual depositing the evidence was present. Although various methods of accomplishing this have been proposed, none has gained widespread use due to poor time resolution and weak age correlation. We have developed a method for the estimation of the time since deposition (TSD) of dried bloodstains using UV-VIS spectrophotometric analysis of hemoglobin (Hb) that is based upon its characteristic oxidation chemistry. A detailed study of the Hb Soret band (λmax=412 nm) in aged bloodstains revealed a blue shift (shift to shorter wavelength) as the age of the stain increases. The extent of this shift permits, for the first time, a distinction to be made between bloodstains that were deposited minutes, hours, days and weeks prior to recovery and analysis. The extent of the blue shift was found to be a function of ambient relative humidity and temperature. The method is extremely sensitive, requiring as little as a 1 µl dried bloodstain for analysis. We demonstrate that it might be possible to perform TSD measurements at the crime scene using a portable low-sample-volume spectrophotometer.
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