ArticleClinical and vaccine immunology : CVI2010
Multiplex assay detection of immunoglobulin G antibodies that recognize Giardia intestinalis and Cryptosporidium parvum antigens.
Article in Clinical and vaccine immunology : CVI, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 52 citations in OpenAlex.
- A Randomized Controlled Trial to Assess the Impact of Ceramic Water Filters on Prevention of Diarrhea and Cryptosporidiosis in Infants and Young Children-Western Kenya, 2013.The American journal of tropical medicine and hygiene · 2018Trial
- The impact of school water, sanitation, and hygiene improvements on infectious disease using serum antibody detection.PLoS neglected tropical diseases · 2018Trial
- Use of Serologic Responses against Enteropathogens to Assess the Impact of a Point-of-Use Water Filter: A Randomized Controlled Trial in Western Province, Rwanda.The American journal of tropical medicine and hygiene · 2017Trial
- Multiplex bead assays enable integrated serological surveillance and reveal cross-pathogen vulnerabilities in Zambezia Province, Mozambique.Nature communications · 2025Article
- Development of an enteric pathogen multiplex immunoassay to measure antibody responses in blood and saliva for integrated serology applications.Journal of immunological methods · 2025Article
- Integrated serological surveillance of communicable diseases in the Paraguayan Chaco, 2019.Revista panamericana de salud publica = Pan American journal of public health · 2025Article
- Disease Biomarkers of Giardiasis.Journal of parasitology research · 2022Review
- Precision of Serologic Testing from Dried Blood Spots Using a Multiplex Bead Assay.The American journal of tropical medicine and hygiene · 2021Article
- Reduced Placental Transfer of Antibodies Against a Wide Range of Microbial and Vaccine Antigens in HIV-Infected Women in Mozambique.Frontiers in immunology · 2021Article
- Encapsulation of Low Metronidazole Dose in Poly (D,L-lactide-co-glycolide) (PLGA) Nanoparticles ImprovesInfection & chemotherapy · 2020Article
- Seroprevalence of antibodies against Chlamydia trachomatis and enteropathogens and distance to the nearest water source among young children in the Amhara Region of Ethiopia.PLoS neglected tropical diseases · 2020Article
- Parasitic Infection Surveillance in Mississippi Delta Children.The American journal of tropical medicine and hygiene · 2020Article
- Integrated Cross-Sectional Multiplex Serosurveillance of IgG Antibody Responses to Parasitic Diseases and Vaccines in Coastal Kenya.The American journal of tropical medicine and hygiene · 2020Article
- High-throughput malaria serosurveillance using a one-step multiplex bead assay.Malaria journal · 2019Article
- Enteropathogen antibody dynamics and force of infection among children in low-resource settings.eLife · 2019Article
- Human Seroprevalence to 11 Zoonotic Pathogens in the U.S. Arctic, Alaska.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2019Article
- Giardia and Cryptosporidium antibody prevalence and correlates of exposure among Alaska residents, 2007-2008.Epidemiology and infection · 2018Article
- Practical Guidance for Clinical Microbiology Laboratories: Laboratory Diagnosis of Parasites from the Gastrointestinal TractClinical microbiology reviews · 2018Review
- Characterizing Reactivity toThe American journal of tropical medicine and hygiene · 2017Article
- Measuring changes in transmission of neglected tropical diseases, malaria, and enteric pathogens from quantitative antibody levels.PLoS neglected tropical diseases · 2017Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giardiasis and cryptosporidiosis are common enteric parasitic diseases that have similar routes of transmission. In this work, we have identified epitopes within the Giardia variant-specific surface protein (VSP) sequences that are recognized by IgG antibodies from 13 of 14 (93%) sera from patients with stool-confirmed giardiasis. The conserved epitopes are shared among VSPs from both of the assemblages that commonly infect humans, and they are likely to be structural, as both sodium dodecyl sulfate treatment and dithiothreitol reduction decrease antibody recognition. In a multiplex bead assay (MBA), we used three VSP fragments from an assemblage A Giardia strain, three VSP fragments from assemblage B strains, and the α-1 giardin structural antigen to detect IgG antibodies to Giardia and used the recombinant 17- and 27-kDa antigens to simultaneously detect IgG antibodies to Cryptosporidium. The MBA differentiated between sera from Giardia and Cryptosporidium outbreaks and also identified a giardiasis outbreak that may have included cryptosporidiosis cases. Approximately 40% of cryptosporidiosis outbreak samples had high MBA responses for both the 27- and 17-kDa antigens, while <10% of nonoutbreak and giardiasis outbreak samples had high responses. At least 60% of giardiasis outbreak samples were positive for antibodies to multiple Giardia antigens, while ≤12% of nonoutbreak samples and samples from U.S. and British Columbia cryptosporidiosis outbreaks met our definition for Giardia seropositivity. A MBA using multiple parasite antigens may prove useful in the epidemiologic analysis of future waterborne or food-borne outbreaks of diarrheal disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.