ArticleNucleic acids research2010
Why barcode? High-throughput multiplex sequencing of mitochondrial genomes for molecular systematics.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed.
- 2,366 new mitochondrial genomes with preliminary identification and phylogeny of >5,500 putative species of beetles.Scientific data · 2026Article
- The complete mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2026Article
- The first complete mitochondrial genome sequences of an ancient orphan evolutionary lineage of Eumolpinae leaf beetles endemic to the South Pacific.BMC genomics · 2025Article
- A Comparative Analysis and Limited Phylogenetic Implications of Mitogenomes in Infraorder-Level Diptera.International journal of molecular sciences · 2025Article
- Population Genetics, Demographic History, and Potential Distributions of the New Important PestsInsects · 2025Article
- Evolutionary Analysis of Hypoderma Pantholopsum in Tibetan Antelopes on the Qinghai-Tibetan Plateau.Acta parasitologica · 2025Article
- The first complete mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2025Article
- The Complete Mitochondrial Genomes ofCurrent issues in molecular biology · 2024Article
- Unravelling the Molecular Identity of Bulgarian Jumping Plant Lice of the Family Aphalaridae (Hemiptera: Psylloidea).Insects · 2024Article
- Article
- The Systematics of the Trematoda.Advances in experimental medicine and biology · 2024Review
- Nanopore adaptive sampling for targeted mitochondrial genome sequencing and bloodmeal identification in hematophagous insects.Parasites & vectors · 2023Article
- Article
- First mitochondrial genome of subfamily Julodinae (Coleoptera, Buprestidae) with its phylogenetic implications.ZooKeys · 2023Article
- Mitochondrial Genomes Provide New Phylogenetic and Evolutionary Insights into Psilidae (Diptera: Brachycera).Insects · 2022Article
- Phylogenetic Position of the GenusInsects · 2021Article
- Next-Generation Sequencing and Its Impacts on Entomological Research in Ecology and Evolution.Neotropical entomology · 2021Review
- Understanding the molecular evolution of tiger diversity through DNA barcoding marker ND4 and NADH dehydrogenase complex using computational biology.Genes & genomics · 2021Article
- Flightlessness in insects enhances diversification and determines assemblage structure across whole communities.Proceedings. Biological sciences · 2021Article
- Assembling mitogenome of Himalayan Black Bear (U. t. laniger) from low depth reads and its application in drawing phylogenetic inferences.Scientific reports · 2021Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Mitochondrial genome sequences are important markers for phylogenetics but taxon sampling remains sporadic because of the great effort and cost required to acquire full-length sequences. Here, we demonstrate a simple, cost-effective way to sequence the full complement of protein coding mitochondrial genes from pooled samples using the 454/Roche platform. Multiplexing was achieved without the need for expensive indexing tags ('barcodes'). The method was trialled with a set of long-range polymerase chain reaction (PCR) fragments from 30 species of Coleoptera (beetles) sequenced in a 1/16th sector of a sequencing plate. Long contigs were produced from the pooled sequences with sequencing depths ranging from ∼10 to 100× per contig. Species identity of individual contigs was established via three 'bait' sequences matching disparate parts of the mitochondrial genome obtained by conventional PCR and Sanger sequencing. This proved that assembly of contigs from the sequencing pool was correct. Our study produced sequences for 21 nearly complete and seven partial sets of protein coding mitochondrial genes. Combined with existing sequences for 25 taxa, an improved estimate of basal relationships in Coleoptera was obtained. The procedure could be employed routinely for mitochondrial genome sequencing at the species level, to provide improved species 'barcodes' that currently use the cox1 gene only.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.