ArticleNucleic acids research2011
The translational response of the human mdm2 gene in HEK293T cells exposed to rapamycin: a role for the 5'-UTRs.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Article
- Preinitiation Complex Loading onto mRNAs with Long versus Short 5' TLs.International journal of molecular sciences · 2022Article
- Cap-independent translation ensures mTOR expression and function upon protein synthesis inhibition.RNA (New York, N.Y.) · 2017Article
- What Is the Impact of mRNA 5' TL Heterogeneity on Translational Start Site Selection and the Mammalian Cellular Phenotype?Frontiers in genetics · 2016Review
- The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype.BMC genomics · 2015Article
- Alternative RNA structure-coupled gene regulations in tumorigenesis.International journal of molecular sciences · 2014Review
- mTOR inhibitors blunt the p53 response to nucleolar stress by regulating RPL11 and MDM2 levels.Cancer biology & therapy · 2014Article
- Fingerprints of a message: integrating positional information on the transcriptome.Frontiers in cell and developmental biology · 2014Article
- Article
- Post-transcriptional regulation in cancer progression : Microenvironmental control of alternative splicing and translation.Journal of cell communication and signaling · 2012Article
- Alternative splicing within the elk-1 5' untranslated region serves to modulate initiation events downstream of the highly conserved upstream open reading frame 2.Molecular and cellular biology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polysomal messenger RNA (mRNA) populations change rapidly in response to alterations in the physiological status of the cell. For this reason, translational regulation, mediated principally at the level of initiation, plays a key role in the maintenance of cellular homeostasis. In an earlier translational profiling study, we followed the impact of rapamycin on polysome re-seeding. Despite the overall negative effect on transcript recruitment, we nonetheless observed that some mRNAs were significantly less affected. Consequently, their relative polysomal occupancy increased in the rapamycin-treated cells. The behaviour of one of these genes, mdm2, has been further analysed. Despite the absence of internal ribosome entry site activity we demonstrate, using a dual reporter assay, that both the reported mdm2 5'-UTRs confer resistance to rapamycin relative to the 5'-UTR of β-actin. This relative resistance is responsive to the downstream targets mTORC1 but did not respond to changes in the La protein, a reported factor acting positively on MDM2 translational expression. Furthermore, extended exposure to rapamycin in the presence of serum increased the steady-state level of the endogenous MDM2 protein. However, this response was effectively reversed when serum levels were reduced. Taken globally, these studies suggest that experimental conditions can dramatically modulate the expressional output during rapamycin exposure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.