ArticleThe Journal of biological chemistry2010
Functional complementation and genetic deletion studies of KirBac channels: activatory mutations highlight gating-sensitive domains.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- An ancestral glutamate receptor mediates cell volume regulation during high KThe Journal of biological chemistry · 2026Article
- Gating and modulation of an inward-rectifier potassium channel.The Journal of general physiology · 2023Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Water Accessibility Refinement of the Extended Structure of KirBac1.1 in the Closed State.Frontiers in molecular biosciences · 2021Article
- Unexpected Gating Behaviour of an Engineered Potassium Channel Kir.Frontiers in molecular biosciences · 2021Article
- The Lipid Activation Mechanism of a Transmembrane Potassium Channel.Journal of the American Chemical Society · 2020Article
- Conduction through a narrow inward-rectifier KThe Journal of general physiology · 2019Article
- Review
- Solution-Based Single-Molecule FRET Studies of K(+) Channel Gating in a Lipid Bilayer.Biophysical journal · 2016Article
- Modular Design of the Selectivity Filter Pore Loop in a Novel Family of Prokaryotic 'Inward Rectifier' (NirBac) channels.Scientific reports · 2015Article
- Molecular Dynamics Simulations of KirBac1.1 Mutants Reveal Global Gating Changes of Kir Channels.Journal of chemical information and modeling · 2015Article
- Tethered protein display identifies a novel Kir3.2 (GIRK2) regulator from protein scaffold libraries.ACS chemical neuroscience · 2014Article
- Control of KirBac3.1 potassium channel gating at the interface between cytoplasmic domains.The Journal of biological chemistry · 2014Article
- Decomposition of slide helix contributions to ATP-dependent inhibition of Kir6.2 channels.The Journal of biological chemistry · 2013Article
- Growth of large and highly ordered 2D crystals of a K⁺ channel, structural role of lipidic environment.Biophysical journal · 2013Article
- Functional characterization and determination of the physiological role of a calcium-dependent potassium channel from cyanobacteria.Plant physiology · 2013Article
- Article
- Using yeast to study potassium channel function and interactions with small molecules.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Structural rearrangements underlying ligand-gating in Kir channels.Nature communications · 2012Article
- Structure of a KirBac potassium channel with an open bundle crossing indicates a mechanism of channel gating.Nature structural & molecular biology · 2012Article
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Authors and funding
12 authors.
Funding
Abstract
The superfamily of prokaryotic inwardly rectifying (KirBac) potassium channels is homologous to mammalian Kir channels. However, relatively little is known about their regulation or about their physiological role in vivo. In this study, we have used random mutagenesis and genetic complementation in K(+)-auxotrophic Escherichia coli and Saccharomyces cerevisiae to identify activatory mutations in a range of different KirBac channels. We also show that the KirBac6.1 gene (slr5078) is necessary for normal growth of the cyanobacterium Synechocystis PCC6803. Functional analysis and molecular dynamics simulations of selected activatory mutations identified regions within the slide helix, transmembrane helices, and C terminus that function as important regulators of KirBac channel activity, as well as a region close to the selectivity filter of KirBac3.1 that may have an effect on gating. In particular, the mutations identified in TM2 favor a model of KirBac channel gating in which opening of the pore at the helix-bundle crossing plays a far more important role than has recently been proposed.
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