ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2011
In situ detection of active transglutaminases for keratinocyte type (TGase 1) and tissue type (TGase 2) using fluorescence-labeled highly reactive substrate peptides.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 40 citations in OpenAlex.
- Human Transglutaminases: Updated Insights into Activation Mechanisms, Allosteric Regulation and Disease.International journal of molecular sciences · 2026Review
- Development of peptide-based biosensors for detecting cross-linking and deamidation activities of transglutaminases.Amino acids · 2023Article
- Transglutaminase-2: Nature's Glue in Lung Fibrosis?American journal of respiratory cell and molecular biology · 2021Article
- Biocatalysis by Transglutaminases: A Review of Biotechnological Applications.Micromachines · 2018Review
- Isozyme-specific comprehensive characterization of transglutaminase-crosslinked substrates in kidney fibrosis.Scientific reports · 2018Article
- Global identification and analysis of isozyme-specific possible substrates crosslinked by transglutaminases using substrate peptides in mouse liver fibrosis.Scientific reports · 2017Article
- Transglutaminase activity is decreased in large arteries from hypertensive rats compared with normotensive controls.American journal of physiology. Heart and circulatory physiology · 2015Article
- Article
- Variations in both TG1 and TG2 isozyme-specific in situ activities and protein expressions during mouse embryonic development.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Niche-specific requirement for hyphal wall protein 1 in virulence of Candida albicans.PloS one · 2013Article
- Vena cava and aortic smooth muscle cells express transglutaminases 1 and 4 in addition to transglutaminase 2.American journal of physiology. Heart and circulatory physiology · 2012Article
- Cellular functions of tissue transglutaminase.International review of cell and molecular biology · 2012Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transglutaminase is a calcium-dependent enzyme that posttranslationally modifies proteins by cross-linking between glutamine and lysine residues or attachment of a primary amine to specific polypeptide-bound glutamine residues. Eight isozymes play essential roles in various mammalian biological processes. The authors have recently identified 12–amino acid preferred substrate peptide sequences that are highly reactive and act in an isozyme-specific manner. In this study, a rapid, isozyme-specific, and sensitive detection of active keratinocyte type (TGase 1) and tissue type (TGase 2) was successful using fluorescence-labeled peptides. This procedure involved using whole-body sections of a mouse to extensively analyze the tissue distribution of both enzymes that revealed clearly distinct patterns. Strong active TGase 1 was observed in epithelial tissues such as tongue, developing teeth, forestomach, and skin epidermis. Significantly active TGase 2 was observed in various types of tissues as predicted and at particularly higher levels in the intestinal mucosa, muscle membrane, and whole veins in the liver. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
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