ArticlePlant physiology2010
Nontransgenic genome modification in plant cells.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 184 citations in OpenAlex.
- Plant viral suppressors HcPro and P19 inhibit CRISPR-Cas13-mediated transcript interference.Stress biology · 2026Article
- From Gene Knockouts to Genome Remodeling: Large DNA Fragment Deletion Technologies in Plants.Plants (Basel, Switzerland) · 2026Review
- Virus-induced gene editing free from tissue culture.Nature plants · 2025Review
- Virus-induced genome editing using a miniature CRISPR system.Nature plants · 2025Article
- Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis.Nature plants · 2025Article
- Viral-Based Gene Editing System for Nutritional Improvement of Fructan Content in Lettuce.International journal of molecular sciences · 2025Article
- Advancements in genome editing tools for genetic studies and crop improvement.Frontiers in plant science · 2024Review
- The R2R3-MYB transcription factor EVER controls the emission of petunia floral volatiles by regulating epicuticular wax biosynthesis in the petal epidermis.The Plant cell · 2023Article
- Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update.Plants (Basel, Switzerland) · 2023Review
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Tools and targets: The dual role of plant viruses in CRISPR-Cas genome editing.The plant genome · 2023Review
- Review
- Review of recent advances in post-harvest techniques for tropical cut flowers and future prospects:Frontiers in plant science · 2023Review
- CRISPR/Cas9 is a powerful tool for precise genome editing of legume crops: a review.Molecular biology reports · 2022Review
- Tobacco Rattle Virus as a Tool for Rapid Reverse-Genetics Screens and Analysis of Gene Function inPlants (Basel, Switzerland) · 2022Article
- Genome Editing: A Promising Approach for Achieving Abiotic Stress Tolerance in Plants.International journal of genomics · 2022Review
- Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin.International journal of molecular sciences · 2021Article
- An Outlook on Global Regulatory Landscape for Genome-Edited Crops.International journal of molecular sciences · 2021Review
- Plant viral vectors: expanding the possibilities of precise gene editing in plant genomes.Plant cell reports · 2021Article
- Plant Viruses: From Targets to Tools for CRISPR.Viruses · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zinc finger nucleases (ZFNs) are a powerful tool for genome editing in eukaryotic cells. ZFNs have been used for targeted mutagenesis in model and crop species. In animal and human cells, transient ZFN expression is often achieved by direct gene transfer into the target cells. Stable transformation, however, is the preferred method for gene expression in plant species, and ZFN-expressing transgenic plants have been used for recovery of mutants that are likely to be classified as transgenic due to the use of direct gene-transfer methods into the target cells. Here we present an alternative, nontransgenic approach for ZFN delivery and production of mutant plants using a novel Tobacco rattle virus (TRV)-based expression system for indirect transient delivery of ZFNs into a variety of tissues and cells of intact plants. TRV systemically infected its hosts and virus ZFN-mediated targeted mutagenesis could be clearly observed in newly developed infected tissues as measured by activation of a mutated reporter transgene in tobacco (Nicotiana tabacum) and petunia (Petunia hybrida) plants. The ability of TRV to move to developing buds and regenerating tissues enabled recovery of mutated tobacco and petunia plants. Sequence analysis and transmission of the mutations to the next generation confirmed the stability of the ZFN-induced genetic changes. Because TRV is an RNA virus that can infect a wide range of plant species, it provides a viable alternative to the production of ZFN-mediated mutants while avoiding the use of direct plant-transformation methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.