ArticleThe Journal of cell biology2010
Imaging proprotein convertase activities and their regulation in the implanting mouse blastocyst.
Article in The Journal of cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Analysis of novel domain-specific mutations in the zebrafishJournal of genetics · 2018Article
- Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling.Development (Cambridge, England) · 2018Article
- Article
- Pcsk5 is required in the early cranio-cardiac mesoderm for heart development.BMC developmental biology · 2017Article
- A Molecular Sensor To Characterize Arenavirus Envelope Glycoprotein Cleavage by Subtilisin Kexin Isozyme 1/Site 1 Protease.Journal of virology · 2016Article
- PC7 and the related proteases Furin and Pace4 regulate E-cadherin function during blastocyst formation.The Journal of cell biology · 2015Article
- The Dynamics of Morphogenesis in the Early Mouse Embryo.Cold Spring Harbor perspectives in biology · 2014Review
- Expression and localization of nodal in bovine oviduct and uterus during different functional stages of oestrus cycle and pregnancy.Histochemistry and cell biology · 2013Article
- The biology and therapeutic targeting of the proprotein convertases.Nature reviews. Drug discovery · 2012Review
- Dynamics of anterior-posterior axis formation in the developing mouse embryo.Nature communications · 2012Article
- On the cutting edge of proprotein convertase pharmacology: from molecular concepts to clinical applications.Biomolecular concepts · 2011Article
- The microenvironment patterns the pluripotent mouse epiblast through paracrine Furin and Pace4 proteolytic activities.Genes & development · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Axis formation and allocation of pluripotent progenitor cells to the germ layers are governed by the TGF-β-related Nodal precursor and its secreted proprotein convertases (PCs) Furin and Pace4. However, when and where Furin and Pace4 first become active have not been determined. To study the distribution of PCs, we developed a novel cell surface-targeted fluorescent biosensor (cell surface-linked indicator of proteolysis [CLIP]). Live imaging of CLIP in wild-type and Furin- and Pace4-deficient embryonic stem cells and embryos revealed that Furin and Pace4 are already active at the blastocyst stage in the inner cell mass and can cleave membrane-bound substrate both cell autonomously and nonautonomously. CLIP was also cleaved in the epiblast of implanted embryos, in part by a novel activity in the uterus that is independent of zygotic Furin and Pace4, suggesting a role for maternal PCs during embryonic development. The unprecedented sensitivity and spatial resolution of CLIP opens exciting new possibilities to elucidate PC functions in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.