ArticleProceedings of the National Academy of Sciences of the United States of America2010
Structural basis for the autoprocessing of zinc metalloproteases in the thermolysin family.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 56 citations in OpenAlex.
- A halophilic metalloprotease fromFrontiers in microbiology · 2025Article
- Copper Forms a PPII Helix-Like Structure with the Catalytic Domains of Bacterial Zinc Metalloproteases.Inorganic chemistry · 2023Article
- Protein sociology of ProA, Mip and other secreted virulence factors at theFrontiers in cellular and infection microbiology · 2023Review
- Quorum-Sensing Regulator OpaR Directly Represses Seven Protease Genes inFrontiers in microbiology · 2020Article
- Characterization of a New M4 Metalloprotease With Collagen-Swelling Ability From MarineFrontiers in microbiology · 2020Article
- An experiment-informed signal transduction model for the role of the Staphylococcus aureus MecR1 protein in β-lactam resistance.Scientific reports · 2019Article
- YpeB dimerization may be required to stabilize SleB for effective germination of Bacillus anthracis spores.BMC microbiology · 2019Article
- Functional characterization of a subtilisin-like serine protease fromThe Journal of biological chemistry · 2019Article
- Towards functional characterization of archaeal genomic dark matter.Biochemical Society transactions · 2019Review
- Tripeptides From Casein Are Signal Molecules to Induce the Expression of the Extracellular Protease MCP-01 Gene in Marine BacteriumFrontiers in microbiology · 2019Article
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- Preparation and functional evaluation of collagen oligopeptide-rich hydrolysate from fish skin with the serine collagenolytic protease from Pseudoalteromonas sp. SM9913.Scientific reports · 2017Article
- Evidence for Cleavage of the Metalloprotease Vsm fromFrontiers in microbiology · 2016Article
- A novel mechanism of latency in matrix metalloproteinases.The Journal of biological chemistry · 2015Article
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- Role of YpeB in cortex hydrolysis during germination of Bacillus anthracis spores.Journal of bacteriology · 2014Article
- A sporulation-specific, sigF-dependent protein, SspA, affects septum positioning in Streptomyces coelicolor.Molecular microbiology · 2014Article
- A novel family of soluble minimal scaffolds provides structural insight into the catalytic domains of integral membrane metallopeptidases.The Journal of biological chemistry · 2013Article
- Structure of gentlyase, the neutral metalloprotease of Paenibacillus polymyxa.Acta crystallographica. Section D, Biological crystallography · 2013Article
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Authors and funding
11 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Thermolysin-like proteases (TLPs), a large group of zinc metalloproteases, are synthesized as inactive precursors. TLPs with a long propeptide (∼200 residues) undergo maturation following autoprocessing through an elusive molecular mechanism. We report the first two crystal structures for the autoprocessed complexes of a typical TLP, MCP-02. In the autoprocessed complex, Ala205 shifts upward by 33 Å from the previously covalently linked residue, His204, indicating that, following autocleavage of the peptide bond between His204 and Ala205, a large conformational change from the zymogen to the autoprocessed complex occurs. The eight N-terminal residues (residues Ala205-Gly212) of the catalytic domain form a new β-strand, nestling into two other β-strands. Simultaneously, the apparent T(m) of the autoprocessed complex increases 20 °C compared to that of the zymogen. The stepwise degradation of the propeptide begins with two sequential cuttings at Ser49-Val50 and Gly57-Leu58, which lead to the disassembly of the propeptide and the formation of mature MCP-02. Our findings give new insights into the molecular mechanism of TLP maturation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.