ArticleBiomedical engineering online2010
Non-viral eNOS gene delivery and transfection with stents for the treatment of restenosis.
Article in Biomedical engineering online, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- Endothelial Dysfunction in Atherosclerosis: Experimental Models and Therapeutics.Biomaterials research · 2025Review
- Lipid Nanoparticles for Nucleic Acid Delivery to Endothelial Cells.Pharmaceutical research · 2023Review
- The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model.Biomedical engineering online · 2021Article
- The Potential Impact and Timeline of Engineering on Congenital Interventions.Pediatric cardiology · 2020Review
- RNA-Eluting Surfaces for the Modulation of Gene Expression as A Novel Stent Concept.Pharmaceuticals (Basel, Switzerland) · 2017Article
- Drug- and Gene-eluting Stents for Preventing Coronary Restenosis.Chonnam medical journal · 2017Review
- The role of atorvastatin on the restenosis process post-PTA in a diabetic rabbit model.BMC cardiovascular disorders · 2016Article
- Novel Fabrication of MicroRNA Nanoparticle-Coated Coronary Stent for Prevention of Post-Angioplasty Restenosis.Korean circulation journal · 2016Article
- Vascular gene transfer from metallic stent surfaces using adenoviral vectors tethered through hydrolysable cross-linkers.Journal of visualized experiments : JoVE · 2014Article
- Nanoparticle drug- and gene-eluting stents for the prevention and treatment of coronary restenosis.Theranostics · 2014Review
- Percutaneous approaches for efficient cardiac gene delivery.Journal of cardiovascular translational research · 2013Review
- Restenosis after PCI. Part 2: prevention and therapy.Nature reviews. Cardiology · 2011Review
- Restenosis after PCI. Part 1: pathophysiology and risk factors.Nature reviews. Cardiology · 2011Review
- Endovascular Gene Delivery from a Stent Platform: Gene- Eluting Stents.Angiology: open accessArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn this study, we have examined local non-viral gene delivery, transfection, and therapeutic efficacy of endothelial nitric oxide synthase (eNOS) encoding plasmid DNA administered using coated stents in a rabbit iliac artery restenosis model.
methodsLipopolyplexes (LPPs) with eNOS expressing plasmid DNA were immobilized on stainless steel stents using poly(D,L-lactide-co-glycolide) (PLGA) and type B gelatin coatings. The gene-eluting stents were implanted bilaterally in the denuded iliac arteries and eNOS transfection and therapeutic efficacy were examined 14 days after implantation.
resultsThe results show that non-viral lipopolyplex-coated stents can efficiently tranfect eNOS locally in the arterial lumen assessed by PCR and ELISA. Human eNOS ELISA levels were significantly raised 24 hours after transfection compared to controls (125 pg eNOS compared to <50 pg for all controls including naked DNA). Local eNOS production suppressed smooth muscle cell proliferation and promoted re-endothelialization of the artery showing a significant reduction in restenosis of 1.75 neointima/media ratio for stents with lipoplexes encoding eNOS compared with 2.3 neointima/media ratio for stents with lipoplexes encosing an empty vector.
conclusionsThese results support the hypothesis that a potent non-viral gene vector encoding for eNOS coated onto a stent can inhibit restenosis through inhibition of smooth muscle cell growth and promotion of a healthy endothelium.
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