ArticleJournal of proteome research2010
Online nanoflow RP-RP-MS reveals dynamics of multicomponent Ku complex in response to DNA damage.
Article in Journal of proteome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
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- Regulator of Chromosome Condensation 2 Modulates Cell Cycle Progression, Tumorigenesis, and Therapeutic Resistance.Frontiers in molecular biosciences · 2020Review
- Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes.Current protocols in protein science · 2019Article
- Advances in microscale separations towards nanoproteomics applications.Journal of chromatography. A · 2017Review
- The human DNA ends proteome uncovers an unexpected entanglement of functional pathways.Nucleic acids research · 2016Article
- The human embryonic stem cell proteome revealed by multidimensional fractionation followed by tandem mass spectrometry.Proteomics · 2015Article
- A fast workflow for identification and quantification of proteomes.Molecular & cellular proteomics : MCP · 2013Article
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
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- Proteomic analysis demonstrates activator- and chromatin-specific recruitment to promoters.The Journal of biological chemistry · 2012Article
- DNA ends alter the molecular composition and localization of Ku multicomponent complexes.Molecular & cellular proteomics : MCP · 2012Article
- Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins.Nature · 2012Article
- Nanoflow low pressure high peak capacity single dimension LC-MS/MS platform for high-throughput, in-depth analysis of mammalian proteomes.Analytical chemistry · 2012Article
- Systematic comparison of fractionation methods for in-depth analysis of plasma proteomes.Journal of proteome research · 2012Article
- Multi-edge gene set networks reveal novel insights into global relationships between biological themes.PloS one · 2012Article
- Sub1 and RPA associate with RNA polymerase II at different stages of transcription.Molecular cell · 2011Article
- Online nanoflow multidimensional fractionation for high efficiency phosphopeptide analysis.Molecular & cellular proteomics : MCP · 2011Article
- Online nanoflow reversed phase-strong anion exchange-reversed phase liquid chromatography-tandem mass spectrometry platform for efficient and in-depth proteome sequence analysis of complex organisms.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Tandem affinity purification (TAP) coupled with mass spectrometry has become the technique of choice for characterization of multicomponent protein complexes. While current TAP protocols routinely provide high yield and specificity for proteins expressed under physiologically relevant conditions, analytical figures of merit required for efficient and in-depth LC-MS analysis remain unresolved. Here we implement a multidimensional chromatography platform, based on two stages of reversed-phase (RP) separation operated at high and low pH, respectively. We compare performance metrics for RP-RP and SCX-RP for the analysis of complex peptide mixtures derived from cell lysate, as well as protein complexes purified via TAP. Our data reveal that RP-RP fractionation outperforms SCX-RP primarily due to increased peak capacity in the first dimension separation. We integrate this system with miniaturized LC assemblies to achieve true online fractionation at low (≤5 nL/min) effluent flow rates. Stable isotope labeling is used to monitor the dynamics of the multicomponent Ku protein complex in response to DNA damage induced by γ radiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.