ArticleTransgenic research2011
Generation of antibody- and B cell-deficient pigs by targeted disruption of the J-region gene segment of the heavy chain locus.
Article in Transgenic research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Generation of IgMScientific reports · 2025Article
- Immune characterization of a xenogeneic human lung cross-circulation support system.Science advances · 2023Article
- Comparative Aspects of Immunoglobulin Gene Rearrangement Arrays in Different Species.Frontiers in immunology · 2022Review
- Unraveling the role of B cells in the pathogenesis of an oncogenic avian herpesvirus.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Genome-editing technologies to improve research, reproduction, and production in pigs.Molecular reproduction and development · 2017Review
- Respiratory nanoparticle-based vaccines and challenges associated with animal models and translation.Journal of controlled release : official journal of the Controlled Release Society · 2015Review
- Current progress of genetically engineered pig models for biomedical research.BioResearch open access · 2014Review
- The porcine innate immune system: an update.Developmental and comparative immunology · 2014Review
- Article
- Use of single stranded targeting DNA or negative selection does not further increase the efficiency of a GGTA1 promoter trap.Journal of molecular cloning & genetic recombination · 2013Article
- Expression of recombinant antibodies.Frontiers in immunology · 2013Article
- Completion of the swine genome will simplify the production of swine as a large animal biomedical model.BMC medical genomics · 2012Article
- Article
- Linkage haplotype for allotypic variants of porcine IgA and IgG subclass genes.Immunogenetics · 2012Article
- Article
- Precision editing of large animal genomes.Advances in genetics · 2012Review
- Efficient immunoglobulin gene disruption and targeted replacement in rabbit using zinc finger nucleases.PloS one · 2011Article
- An activated immune and inflammatory response targets the pancreas of newborn pigs with cystic fibrosis.Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] · 2011Article
- Genetic modifications of pigs for medicine and agriculture.Molecular reproduction and developmentReview
Corrections and comments
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Authors and funding
20 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A poly(A)-trap gene targeting strategy was used to disrupt the single functional heavy chain (HC) joining region (J(H)) of swine in primary fibroblasts. Genetically modified piglets were then generated via somatic cell nuclear transfer (SCNT) and bred to yield litters comprising J(H) wild-type littermate (+/+), J(H) heterozygous knockout (±) and J(H) homozygous knockout (-/-) piglets in the expected Mendelian ratio of 1:2:1. There are only two other targeted loci previously published in swine, and this is the first successful poly(A)-trap strategy ever published in a livestock species. In either blood or secondary lymphoid tissues, flow cytometry, RT-PCR and ELISA detected no circulating IgM(+) B cells, and no transcription or secretion of immunoglobulin (Ig) isotypes, respectively in J(H) -/- pigs. Histochemical and immunohistochemical (IHC) studies failed to detect lymph node (LN) follicles or CD79α(+) B cells, respectively in J(H) -/- pigs. T cell receptor (TCR)(β) transcription and T cells were detected in J(H) -/- pigs. When reared conventionally, J(H) -/- pigs succumbed to bacterial infections after weaning. These antibody (Ab)- and B cell-deficient pigs have significant value as models for both veterinary and human research to discriminate cellular and humoral protective immunity to infectious agents. Thus, these pigs may aid in vaccine development for infectious agents such as the pandemic porcine reproductive and respiratory syndrome virus (PRRSV) and H1N1 swine flu. These pigs are also a first significant step towards generating a pig that expresses fully human, antigen-specific polyclonal Ab to target numerous incurable infectious diseases with high unmet clinical need.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.