ArticleCurrent microbiology2011
Construction and characterization of Escherichia coli whole-cell biosensors for toluene and related compounds.
Article in Current microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- A Versatile High Throughput Screening Platform for Plant Metabolic Engineering Highlights the Major Role ofFrontiers in plant science · 2020Article
- Characterization of a promiscuous cadmium and arsenic resistance mechanism in Thermus thermophilus HB27 and potential application of a novel bioreporter system.Microbial cell factories · 2018Article
- Development of a 2-Nitrobenzoate-Sensing Bioreporter Based on an Inducible Gene Cluster.Frontiers in microbiology · 2018Article
- The Application of Whole Cell-Based Biosensors for Use in Environmental Analysis and in Medical Diagnostics.Sensors (Basel, Switzerland) · 2017Review
- New family of biosensors for monitoring BTX in aquatic and edaphic environments.Microbial biotechnology · 2016Article
- Synthetic biology expands chemical control of microorganisms.Current opinion in chemical biology · 2015Review
- Design and Construction of a Whole Cell Bacterial 4-Hydroxyphenylacetic Acid and 2-Phenylacetic Acid Bioassay.Frontiers in bioengineering and biotechnology · 2015Article
- New quaternary ammonium camphor derivatives and their antiviral activity, genotoxic effects and cytotoxicity.Bioorganic & medicinal chemistry · 2013Article
- Genetic sensor for strong methylating compounds.ACS synthetic biology · 2013Article
- Genetically modified whole-cell bioreporters for environmental assessment.Ecological indicators · 2013Article
- Construction and application of an Escherichia coli bioreporter for aniline and chloroaniline detection.Journal of industrial microbiology & biotechnology · 2012Article
- Increasing signal specificity of the TOL network of Pseudomonas putida mt-2 by rewiring the connectivity of the master regulator XylR.PLoS genetics · 2012Article
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Authors and funding
4 authors.
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Abstract
The XylR regulatory protein is a transcriptional activator from the TOL plasmid of Pseudomonas putida mt-2 that is involved in the toluene and benzene degradation pathway. Here we describe the construction and laboratory characterization of recombinant biosensors (pGLPX plasmids) based on XylR and its cognate promoter (Pu). In the pGLPX plasmid, the reporter luc gene is under the control of the Pu promoter. We evaluated the ability of two distinct nucleotide sequences to function as SD elements and improve sensitivity of bioreporting. We also evaluated the effect of introducing the T₂rrnβ terminator on the specificity of the construct. E. coli transformed with pGLPX plasmids were used to sense toluene and its derivatives. The pattern of induction was different for each derivative. In general, more luciferase activity was induced by toluene and benzene than by TNT and DNT at most tested concentrations. The bioluminescence response of the reporter strains to the nitrotoluenes was significantly stronger at lower concentrations (≥ 50 μmol) than at higher concentrations. Our results show that the SD sequence (taaggagg) is crucially important for biosensor sensitivity. The presence of the T₂rrnβ terminator in the bioreporter plasmid prevents nonspecific responses and also reduces biosensor sensitivity upon exposure to inducers. These data suggest that pGLPX strains can be used as whole-cell biosensors to detect toluene and related compounds. Further investigation will be required to optimize the application of pGLPX biosensors.
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