ArticleIntegrative biology : quantitative biosciences from nano to macro2010
Microfluidic platform for chemotaxis in gradients formed by CXCL12 source-sink cells.
Article in Integrative biology : quantitative biosciences from nano to macro, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
38 citing papers in PubMed, 78 citations in OpenAlex.
- Quantitative Spatiotemporal Analysis of Intracellular Kinase Activity in Metastatic Breast Cancer Cells Using a Microfluidic-Based Lateral Diffusion Assay.Analytical chemistry · 2026Article
- Design Optimization of a 3D Microfluidic Channel System for Biomedical Applications.3D printing and additive manufacturing · 2024Article
- Patterning Neuroepithelial Cell SheetACS biomaterials science & engineering · 2021Article
- Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa.Analytical chemistry · 2020Article
- Beyond Cell Motility: The Expanding Roles of Chemokines and Their Receptors in Malignancy.Frontiers in immunology · 2020Review
- Plug-and-Play In Vitro Metastasis System toward Recapitulating the Metastatic Cascade.Scientific reports · 2019Article
- Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption.Micromachines · 2019Article
- Engineering of vascularized 3D cell constructs to model cellular interactions through a vascular network.Biomicrofluidics · 2018Article
- A microfluidic platform for modeling metastatic cancer cell matrix invasion.Biofabrication · 2017Article
- Tumor Microenvironment on a Chip: The Progress and Future Perspective.Bioengineering (Basel, Switzerland) · 2017Review
- Multiscale microenvironmental perturbation of pluripotent stem cell fate and self-organization.Scientific reports · 2017Article
- Transitions from mono- to co- to tri-culture uniquely affect gene expression in breast cancer, stromal, and immune compartments.Biomedical microdevices · 2016Article
- Progress towards understanding heterotypic interactions in multi-culture models of breast cancer.Integrative biology : quantitative biosciences from nano to macro · 2016Review
- In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform.Frontiers in bioengineering and biotechnology · 2016Review
- A Perspective on Studying G-Protein-Coupled Receptor Signaling with Resonance Energy Transfer Biosensors in Living Organisms.Molecular pharmacology · 2015Review
- MicroC(3): an ex vivo microfluidic cis-coculture assay to test chemosensitivity and resistance of patient multiple myeloma cells.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Surface-templated hydrogel patterns prompt matrix-dependent migration of breast cancer cells towards chemokine-secreting cells.Acta biomaterialia · 2015Article
- Article
- Diverse and dynamic sources and sinks in gradient formation and directed migration.Current opinion in cell biology · 2014Review
- Microfluidic source-sink model reveals effects of biophysically distinct CXCL12 isoforms in breast cancer chemotaxis.Integrative biology : quantitative biosciences from nano to macro · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Chemokine CXCL12 promotes CXCR4-dependent chemotaxis of cancer cells to characteristic organs and tissues, leading to metastatic disease. This study was designed to investigate how cells expressing CXCR7 regulate chemotaxis of a separate population of CXCR4 cells under physiologic conditions in which cells are exposed to gradients of CXCL12. We recapitulated a cancer-stroma microenvironment by patterning CXCR4-expressing cancer cells in microchannels at spatially defined positions relative to CXCL12-producing cells and CXCR7-expressing cells. CXCR7 scavenges and degrades CXCL12, which has been proposed to facilitate CXCR4-dependent chemotaxis through a source-sink model. Using the microchannel device, we demonstrated that chemotaxis of CXCR4 cells depended critically on the presence and location of CXCR7 cells (sink) relative to chemokine secreting cells (source). Furthermore, inhibiting CXCR4 on migrating cells or CXCR7 on sink cells blocked CXCR4-dependent chemotaxis toward CXCL12, showing that the device can identify new therapeutic agents that block migration by targeting chemoattractant scavenging receptors. Our system enables efficient chemotaxis under much shallower yet more physiological chemoattractant gradients by generating an in vitro microenvironment where combinations of cellular products may be secreted along with formation of a chemoattractant gradient. In addition to elucidating mechanisms of CXCL-12 mediated chemotaxis, this simple and robust method can be broadly useful for engineering multiple microenvironments to investigate intercellular communication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.